An enlarged largest subunit of Plasmodium falciparum RNA polymerase II defines conserved and variable RNA polymerase domains.
An enlarged largest subunit of Plasmodium falciparum RNA polymerase II defines conserved and variable RNA polymerase domains.
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恶性疟原虫 RNA 聚合酶 II 的放大的最大亚基定义了保守和可变的 RNA 聚合酶结构域。
DOI：
10.1093/nar/17.23.9621
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发表时间：
1989
影响因子：
14.9
通讯作者：
Inselburg,J
中科院分区：
文献类型：
--
作者：
Li,WB;Bzik,DJ;Gu,HM;Tanaka,M;Fox,BA;Inselburg,J
我们从恶性疟原虫中分离出了编码 RNA 聚合酶 II 最大亚基的基因。 RPII 基因在寄生虫的无性红细胞阶段以 9 kb mRNA 的形式表达，并作为位于 3 号染色体上的单拷贝基因存在。恶性疟原虫 RPII 亚基是最大的（2452 个氨基酸）真核 RPII 亚基，并且它包含扩大的可变区，这些可变区清楚地分隔和定义了真核 RPII 最大亚基的 5 个保守区。独特的羧基末端结构域包含短的高度保守的七肽重复结构域，该结构域的 5' 侧与高度分歧的七肽重复结构域结合，并且其 3' 侧由长的羧基末端延伸部分结合。
We have isolated the gene encoding the largest subunit of RNA polymerase II fromPlasmodium falciparum. The RPII gene is expressed in the asexual erythrocytic stages of the parasite as a 9 kb mRNA, and is present as a single copy gene located on chromosome 3. The P.falciparumRPII subunit is the largest (2452 amino acids) eukaryotic RPII subunit, and it contains enlarged variable regions that clearly separate and define five conserved regions of the eukaryotic RPII largest subunits. A distinctive carboxyl-terminal domain contains a short highly conserved heptapeptide repeat domain which is bounded on its 5' side by a highly diverged heptapeptide repeat domain, and is bounded on its 3' side by a long carboxyl-terminal extension.