Mannitol infusion immediately after reperfusion suppresses the development of focal cortical infarction after temporary cerebral ischemia in gerbils.
Mannitol infusion immediately after reperfusion suppresses the development of focal cortical infarction after temporary cerebral ischemia in gerbils.
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DOI：
10.1111/neup.12113
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发表时间：
2014-08
期刊：
影响因子：
--
通讯作者：
Oyanagi K
中科院分区：
文献类型：
--
作者：
Ito U;Hakamata Y;Watabe K;Oyanagi K
前期我们发现，暂时性脑缺血后，肿胀的星形胶质细胞尾足压迫微血管，造成微血管性继发性局灶性脑皮质缺血，导致局灶性梗死。在此，我们检查了血流恢复后立即输注甘露醇是否可以保护大脑皮层免受此类梗塞的发展。如果是这样，输注甘露醇可能会改善血管再灌注治疗的效果。我们选择左颈动脉闭塞后前 10 分钟内的中风阳性动物，进行两次（每次 5 小时），并将它们分为四组：假手术对照组、不治疗组、甘露醇输注组和盐水输注组。对在交叉水平制备的冠状切片的大脑皮层进行光学和电子显微镜研究，其中局灶性梗塞在播散性选择性神经元坏死正在成熟的区域突然发生。进行测量以确定以下内容：（A）血流恢复后 72 小时和 120 小时时所有组的 HE 染色样本中的梗死面积； (B) 对照组以及未治疗组和甘露醇输注组在 0.5、3、5、8、12 和 24 小时时灌注炭黑悬浮液的微血管数量； (C) 星形胶质细胞端足区域； (D) 5小时时拍摄的电子显微镜照片中星形胶质细胞末端足中线粒体的数量。甘露醇组梗塞面积的平均小数分数面积比在72小时和120小时显着降低，与炭黑悬浮液灌注的微血管在3小时、5小时和8小时时的小数分数数比率增加相关，并且与5小时时末足尺寸显着减小有关。暂时性脑缺血血流恢复后立即输注甘露醇，可通过减少足端肿胀，显着缩小大脑皮质局灶性梗死的范围，从而防止微血管受压和瘀滞，从而防止微血管源性继发性局灶性脑缺血。
Previously we found that, after temporary cerebral ischemia, microvasculogenic secondary focal cerebral cortical ischemia occurred, caused by microvascular obstruction due to compression by swollen astrocytic end-feet, resulting in focal infarction. Herein, we examined whether mannitol infusion immediately after restoration of blood flow could protect the cerebral cortex against the development of such an infarction. If so, the infusion of mannitol might improve the results of vascular reperfusion therapy. We selected stroke-positive animals during the first 10 min after left carotid occlusion performed twice with a 5-h interval, and allocated them into four groups: sham-operated control, no-treatment, mannitol-infusion, and saline-infusion groups. Light- and electron-microscopic studies were performed on cerebral cortices of coronal sections prepared at the chiasmatic level, where the focal infarction develops abruptly in the area where disseminated selective neuronal necrosis is maturing. Measurements were performed to determine the following: (A) infarct size in HE-stained specimens from all groups at 72 and 120 h after return of blood flow; (B) number of carbon-black-suspension-perfused microvessels in the control and at 0.5, 3, 5, 8, 12 and 24 h in the no-treatment and mannitol-infusion groups; (C) area of astrocytic end-feet; and (D) number of mitochondria in the astrocytic end-feet in electron microscopic pictures taken at 5 h. The average decimal fraction area ratio of infarct size in the mannitol group was significantly reduced at 72 and 120 h, associated with an increased decimal fraction number ratio of carbon-black-suspension-perfused microvessels at 3, 5 and 8 h, and a marked reduction in the size of the end-feet at 5 h. Mannitol infusion performed immediately after restitution of blood flow following temporary cerebral ischemia remarkably reduced the size of the cerebral cortical focal infarction by decreasing the swelling of the end-feet, thus preventing the microvascular compression and stasis and thereby microvasculogenic secondary focal cerebral ischemia.
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临时[校正]脑缺血会导致星形细胞末端肿胀，从而压缩微丝并导致延迟[校正]局灶性皮质梗塞。
DOI：
10.1038/jcbfm.2010.97
发表时间：
2011-01
期刊：
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism
影响因子：
--
作者：
通讯作者：
--
影响因子：
12.7
作者：
Kuroiwa, T;Mies, G;Ito, U
通讯作者：
Ito, U
影响因子：
6.3
作者：
Lo, ACY;Chen, AYS;Chung, SK
通讯作者：
Chung, SK
影响因子：
48
作者：
Bonati, Leo H.;Jongen, Lisa M.;Engelter, Stefan T.
通讯作者：
Engelter, Stefan T.
影响因子：
2.9
作者：
HANYU, SJ;ITO, U;YOSHIDA, M
通讯作者：
YOSHIDA, M