THE DISTRIBUTION OF DUST IN THE RAT LUNG FOLLOWING ADMINISTRATION BY INHALATION AND BY SINGLE INTRATRACHEAL INSTILLATION
THE DISTRIBUTION OF DUST IN THE RAT LUNG FOLLOWING ADMINISTRATION BY INHALATION AND BY SINGLE INTRATRACHEAL INSTILLATION
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DOI：
10.1016/0013-9351(85)90025-8
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发表时间：
1985-01-01
影响因子：
8.3
通讯作者：
MORGAN, A
中科院分区：
文献类型：
--
作者：
PRITCHARD, JN;HOLMES, A;MORGAN, A
比较了吸入和单次气管内滴注给药后大鼠肺内粉尘的分布。由于各种各样的注入技术，采用平均方法的基础上，从15个实验室在欧洲和美国的调查结果。新技术的发展，定量分布的灰尘在肺内的微观和宏观。通过注入获得的粉尘分布比通过吸入获得的粉尘分布要均匀得多，并且渗透到外围的粉尘很少。研究了改变悬浮液体积、改变悬浮液浓度和向注射器中引入空气的影响。虽然在注射器中使用空气是有益的，但没有任何其他修改对分布有任何改善。吸入和滴入后分布的差异导致结论，后者可能用于确定肺部对不同粉尘的性质和比较反应，但不能用于建立绝对剂量-反应关系。不应给成年大鼠单次滴注1mg纤维或5mg非纤维粉尘。
A comparison was made of the distribution of dust in the rat lung following its administration by inhalation and by a single intratracheal instillation. Due to the wide variety of instillation techniques, an average method was adopted based on the results from a survey of 15 laboratories in Europe and the USA. Novel techniques were developed to quantify the distribution of dust within the lung both microscopically and macroscopically. The distribution of dust obtained by instillation was shown to be much less homogeneous than that obtained by inhalation and penetration to the periphery was minimal. The effects of changing the volume of suspension, changing the concentration of the suspension and introducing air into the syringe were investigated. Although the use of air in the syringe was beneficial, none of the other modifications effected any improvement in the distribution. The difference in distribution following inhalation and instillation leads to the conclusion that the latter may be of use in establishing the nature and comparative response of the lung to different dusts, but not for establishing an absolute dose-response relationship. No > 1 mg of fibros or 5 mg of nonfibros dust should be given in a single instillation to adult rats.