DUAL STAINING OF CORNEAL ENDOTHELIUM WITH TRYPAN BLUE AND ALIZARIN RED-S - IMPORTANCE OF PH FOR THE DYE-LAKE REACTION
DUAL STAINING OF CORNEAL ENDOTHELIUM WITH TRYPAN BLUE AND ALIZARIN RED-S - IMPORTANCE OF PH FOR THE DYE-LAKE REACTION
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DOI：
10.1136/bjo.65.12.815
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发表时间：
1981-01-01
影响因子：
4.1
通讯作者：
HUNT, CJ
中科院分区：
文献类型：
--
作者：
TAYLOR, MJ;HUNT, CJ
用台盼蓝染色和茜素红染色联合评价角膜内皮的完整性提供了一种简单、快速的观察受损细胞和正常细胞的技术；这允许内皮细胞损伤的量化。将茜素红S试剂的pH值调整到4.2对于在细胞间边界获得最佳染色效果很重要，用戊二醛短暂固定可以保持两种染料的染色效果。
Evaluation of corneal endothelial integrity by combined staining with the vital stain trypan blue and the intercellular stain Alizarin Red S provides a simple, quick technique for visualization of damaged and normal cells; this permits the quantification of endothelial cell damage. Adjustment of the pH of the Alizarin Red S reagent to 4.2 is important for optimum dye-laking at the intercellular borders, and brief fixation with glutaraldehyde maintains the staining effect of both dyes.