INVIVO ADMINISTRATION OF ANTIBODY TO MURINE IL-5 RECEPTOR INHIBITS EOSINOPHILIA OF IL-5 TRANSGENIC MICE
INVIVO ADMINISTRATION OF ANTIBODY TO MURINE IL-5 RECEPTOR INHIBITS EOSINOPHILIA OF IL-5 TRANSGENIC MICE
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DOI：
10.1093/intimm/3.2.135
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发表时间：
1991-02-01
影响因子：
4.4
通讯作者：
TAKATSU, K
中科院分区：
文献类型：
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作者：
HITOSHI, Y;YAMAGUCHI, N;TAKATSU, K
我们研究了白细胞介素5（IL-5）及其受体（IL-5 R）在嗜酸性粒细胞生长和分化中的体内作用。当小鼠腹膜内给予IL-5时，5天内在外周血和腹膜腔中观察到嗜酸性粒细胞数量的增加。在IL-5转基因小鼠中观察到嗜酸性粒细胞增多，这些小鼠表现出IL-5的组成性产生。与S-35标记的IL-5的结合试验显示，在嗜酸性粒细胞表面存在两类IL-5结合位点（低亲和力和高亲和力）。使用抗鼠IL-5 R的mAb识别嗜酸性粒细胞上的IL-5 R。当IL-5转基因小鼠被动给予抗鼠IL-5 R mAb时，外周血中可识别的嗜酸性粒细胞的数量在5天内下降到正常水平。抗体治疗还防止了IL-5注射小鼠中嗜酸性粒细胞数量的增加。通过被动给予抗IL-5 mAb也观察到上述实验性嗜酸性粒细胞增多的抑制。体内实验的结果清楚地表明，IL-5在体内嗜酸性粒细胞生成中起重要作用，并且可能直接通过IL-5 R作用于嗜酸性粒细胞或其前体，导致该谱系造血细胞的优先生长。还可以强调，IL-5调节造血细胞（嗜酸性粒细胞）的特定谱系。
We investigated the in vivo role of interleukin 5 (IL-5) and its receptor (IL-5R) in eosinophil growth and differentiation. When mice were administered IL-5 i.p., an increase in the number of eosinophils was observed within 5 days in peripheral blood and the peritoneal cavity. Hypereosinophilia was observed in IL-5 transgenic mice who displayed constitutive production of IL-5. A binding assay with S-35-labeled IL-5 revealed the presence of two classes of IL-5 binding sites (low and high affinity) on the surface of eosinophils. IL-5Rs on eosinophils were recognized using mAbs against murine IL-5R. When the IL-5 transgenic mice were passively administered with anti-murine IL-5R mAbs, the number of recognizable eosinophils in peripheral blood dropped within 5 days to normal levels. The antibody treatment also prevented the increase in the number of eosinophils in IL-5-injected mice. The inhibition of the above experimental eosinophilia was also observed by the passive administration of anti-IL-5 mAb. The results of the in vivo experiments clearly demonstrate that IL-5 plays an essential role in in vivo eosinophilopoiesis and may be acting on eosinophils or their precursors directly through IL-5Rs, resulting in preferential growth of this lineage of hematopoietic cells. It can also be stressed that IL-5 regulates a specific lineage of hematopoietic cells (eosinophils).