MIXED HUMAN IMMUNODEFICIENCY VIRUS (HIV) INFECTION IN AN INDIVIDUAL - DEMONSTRATION OF BOTH HIV TYPE-1 AND TYPE-2 PROVIRAL SEQUENCES BY USING POLYMERASE CHAIN-REACTION
MIXED HUMAN IMMUNODEFICIENCY VIRUS (HIV) INFECTION IN AN INDIVIDUAL - DEMONSTRATION OF BOTH HIV TYPE-1 AND TYPE-2 PROVIRAL SEQUENCES BY USING POLYMERASE CHAIN-REACTION
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DOI：
10.1093/infdis/158.6.1170
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发表时间：
1988-12-01
影响因子：
6.4
通讯作者：
OU, CY
中科院分区：
文献类型：
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作者：
RAYFIELD, M;DECOCK, K;OU, CY
从1987年在西非科特迪瓦阿比让对944人进行的血清阳性率研究中，选择了对人类免疫缺陷病毒1型（HIV-1）和2型（HIV-2）均具有血清反应性的人的血清，通过全病毒（VEIA）酶免疫测定（EIA）对每种病毒进行检测。随后通过两种病毒的类型特异性肽EIA（PEIA）和蛋白质印迹（WB）分析来测试这些血清的HIV-1和HIV-2抗体特异性。在植物血凝素刺激的正常供体PBMC存在下培养来自代表性个体的外周血单核细胞（PBMC）。使用选择性DNA扩增技术聚合酶链反应（PCR）定期监测这些培养物的HIV-1和HIV-2前病毒序列。作为这项研究的结果，我们报告的情况下，一个人的双重反应，通过各种血清学技术，其中HIV-1和HIV-2的前病毒序列通过PCR检测。这是首例确诊的HIV-1和HIV-2混合感染病例。
Sera from persons seroreactive to both human immunodeficiency virus type 1 (HIV-1) and type 2 (HIV-2), by whole-virus (VEIA) enzyme immunoassays (EIAs) for each virus, were selected from a seroprevalence study of 944 persons in Abidjan, Cote d''Ivoire, West Africa, in 1987. These sera were subsequently tested for HIV-1 and HIV-2 antibody specificity by type-specific peptide EIAs (PEIA) and western blot (WB) analysis for both viruses. Peripheral blood monocytes (PBMCs) from representative individuals were cultured in the presence of phytohemagglutinin-stimulated normal donor PBMCs. These cultures were periodically monitored for HIV-1 and HIV-2 proviral sequences by using the selective DNA amplification technique polymerase chain reaction (PCR). As an outgrowth of this study, we report the case of a person dually reactive by various serological techniques in whom proviral sequences from HIV-1 and HIV-2 were detected by PCR. This is the first confirmed case of a mixed HIV-1 and HIV-2 infection in a single individual.