Bovine meniscal tissue exhibits age- and interleukin-1 dose-dependent degradation patterns and composition-function relationships
Bovine meniscal tissue exhibits age- and interleukin-1 dose-dependent degradation patterns and composition-function relationships
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DOI：
10.1002/jor.23096
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发表时间：
2016-05-01
影响因子：
2.8
通讯作者：
Levenston, Marc E.
中科院分区：
文献类型：
--
作者：
Ling, Carrie H-Y;Lai, Janice H.;Levenston, Marc E.
尽管越来越多的证据表明半月板退化是膝骨关节炎发展的早期事件，但关于细胞因子诱导的半月板退化的顺序或功能含义，以及退化如何随年龄变化，我们所知的相对较少。本研究检测了白细胞介素-1 （IL-1）诱导的幼年和成年牛半月板径向中部外植体基质降解的剂量依赖性模式。组织外植体分别在0、1.25、5、20ng/ml重组人IL-1中培养10天。幼年外植体表现出立即和广泛的硫酸氨基聚糖（sGAG）损失和随后的胶原蛋白释放，在4-6天后开始，相对较少的IL-1剂量依赖性。成体外植体对IL-1表现出更分级的反应，sGAG的释放与剂量有关，sGAG的释放比例比幼体外植体低（但绝对释放量更高）。与幼年外植体相比，在10天的培养过程中，成年外植体的胶原蛋白释放量最小。压缩模量和剪切模量反映了外植体组成的变化，两个年龄的外植体都有明显的下降，但幼体组织的相对下降幅度更大。幼年组织的动态模量对外植体sGAG含量的依赖性更强，可能反映了蛋白多糖和胶原组织成分的伴随变化。组织降解的模式表明，像关节软骨一样，半月板蛋白聚糖可能部分保护胶原蛋白免受细胞介导的变性。更详细地了解半月板组织力学与退行性变的功能变化将有助于建立体外培养模型的相关性，并将促进对半月板退行性变如何促进早期骨关节炎整体关节变化的理解。(c) 2015年骨科研究会。Wiley期刊公司出版。[J]中华口腔医学杂志，2016,34(4):591 - 591。
Despite increasing evidence that meniscal degeneration is an early event in the development of knee osteoarthritis, relatively little is known regarding the sequence or functional implications of cytokine-induced meniscal degradation or how degradation varies with age. This study examined dose-dependent patterns of interleukin-1 (IL-1)-induced matrix degradation in explants from the radially middle regions of juvenile and adult bovine menisci. Tissue explants were cultured for 10 days in the presence of 0, 1.25, 5, or 20ng/ml recombinant human IL-1. Juvenile explants exhibited immediate and extensive sulfated glycosaminoglycan (sGAG) loss and subsequent collagen release beginning after 4-6 days, with relatively little IL-1 dose-dependence. Adult explants exhibited a more graded response to IL-1, with dose-dependent sGAG release and a lower fraction of sGAG released (but greater absolute release) than juvenile explants. In contrast to juvenile explants, adult explants exhibited minimal collagen release over the 10-day culture. Compressive and shear moduli reflected the changes in explant composition, with substantial decreases for both ages but a greater relative decrease in juvenile tissue. Dynamic moduli exhibited stronger dependence on explant sGAG content for juvenile tissue, likely reflecting concomitant changes to both proteoglycan and collagen tissue components. The patterns of tissue degradation suggest that, like in articular cartilage, meniscal proteoglycans may partially protect collagen from cell-mediated degeneration. A more detailed view of functional changes in meniscal tissue mechanics with degeneration will help to establish the relevance of in vitro culture models and will advance understanding of how meniscal degeneration contributes to overall joint changes in early stage osteoarthritis. (c) 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:801-811, 2016.