Selenoprotein W was Correlated with the Protective Effect of Selenium on Chicken Myocardial Cells from Oxidative Damage
Selenoprotein W was Correlated with the Protective Effect of Selenium on Chicken Myocardial Cells from Oxidative Damage
复制标题
硒蛋白W与硒对鸡心肌细胞氧化损伤的保护作用相关
DOI：
10.1007/s12011-015-0529-7
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发表时间：
2016-06-01
影响因子：
3.9
通讯作者：
Xu, Shiwen
中科院分区：
文献类型：
--
作者：
Liu, Wei;Yao, Haidong;Xu, Shiwen
Selenium (Se) mainly performs its function through Se-containing proteins. Selenoprotein W (SelW), one member of the selenoprotein family, plays important roles in the normal function of the heart. To investigate the possible relationship between Se and SelW for the regulation of oxidative damage in chicken embryo myocardial cells, we treated myocardial cells with Se and H2O2. Then, the levels of lactate dehydrogenase (LDH) and 3,4-methylenedioxyamphetamine in the culture media, levels of SelW, inflammatory genes NF-κB, tumor necrosis factor (TNF)-α, p53, and the cell cycle were analyzed. Furthermore, the correlation between SelW and the levels of these factors was determined. The results indicated that Se treatment increased the expression of SelW (P< 0.05) and caused a downregulation of p53, NF-κB, and TNF-α (P< 0.05). In contrast, H2O2increased the expression of p53, NF-κB, TNF-α, and LDH (P< 0.05) and induced early cell apoptosis, which was alleviated by treatment with Se. In addition, SelW had a positive correlation with the levels of inflammatory genes investigated. Taken together, our findings suggested that SelW is sensitive to Se levels and oxidative stress, and may play a role in the protective function of Se against oxidative damage and inflammation in chicken myocardial cells.
