Protein Expression Profile of the Mouse Metaphase-II Oocyte
Protein Expression Profile of the Mouse Metaphase-II Oocyte
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DOI：
10.1021/pr800392s
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发表时间：
2008-11-01
影响因子：
4.4
通讯作者：
Zhou, Qi
中科院分区：
文献类型：
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作者：
Ma, Minyue;Guo, Xuejiang;Zhou, Qi
成熟的卵母细胞含有受精、向合子转录的过渡和胚胎发生的开始阶段所需的全部母体蛋白。这些蛋白质中的许多还有待鉴定。本研究采用银染或Pro-Q Diamond染色的小鼠中期II（MII）卵母细胞蛋白质双向电泳（2-DE）技术，对ICR小鼠卵母细胞的蛋白质组和磷酸化蛋白质组进行了研究。共869个蛋白质点，对应于380个独特的蛋白质，被成功地通过质谱鉴定，其中90个蛋白质点，代表53个独特的蛋白质已被染色的Pro-Q钻石，表明他们是在磷酸化的形式。所有鉴定的蛋白质进行了详细的生物信息学注释，并与胚胎干细胞（ESC）蛋白质组进行了比较。根据本研究中产生的蛋白质数据建立了小鼠卵母细胞蛋白质组参考数据库，该数据库可以通过互联网访问（http：//reprod.njmu.edu.cn/2d）。该数据库是迄今为止最详细的小鼠卵母细胞蛋白质组数据库。它应该是有价值的，在扩大我们的知识，在卵子发生，受精和胚胎发育的信号调节，同时揭示表观遗传重编程的潜在机制。
The mature oocyte contains the full complement of maternal proteins required for fertilization, the transition to zygotic transcription, and the beginning stages of embryogenesis. Many of these proteins have yet to be characterized. In this study, two-dimensional electrophoresis (2-DE) of mouse metaphase-II (MII) oocyte proteins, stained with silver staining or Pro-Q Diamond dye, was performed to describe the proteome and phosphoproteome of the mouse oocyte derived from ICR mice. A total of 869 selected protein spots, corresponding to 380 unique proteins, were identified successfully by mass spectrometry, in which 90 protein spots representing 53 unique proteins have been stained with Pro-Q Diamond, indicating that they are in phosphorylated forms. All identified proteins were bioinformatically annotated in detail and compared with the embryonic stem cell (ESC) proteome. A proteome reference database for the mouse oocyte was established from the protein data generated in this study, which can be accessed over the Internet (http://reprod.njmu.edu.cn/2d). This database is the most detailed mouse oocyte proteomic database to date. It should be valuable in expanding our knowledge of the regulation of signaling in oogenesis, fertilization, and embryo development, while revealing potential mechanisms for epigenetic reprogramming.