Nanostimulation: Manipulation of Single Neuron Activity by Juxtacellular Current Injection
Nanostimulation: Manipulation of Single Neuron Activity by Juxtacellular Current Injection
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DOI：
10.1152/jn.00421.2009
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发表时间：
2010-03-01
影响因子：
2.5
通讯作者：
Brecht, Michael
中科院分区：
文献类型：
--
作者：
Houweling, Arthur R.;Doron, Guy;Brecht, Michael
杨建军，李建军，李建军，等。细胞旁电流注射对神经元活动的影响。中国生物医学工程杂志[J] . 32(2): 481 - 481。首次发表于2009年12月2日；doi: 10.1152 / jn.00421.2009。在哺乳动物的大脑中，已经进行了成千上万的单神经元记录研究，但只有不到10个单细胞刺激研究。单细胞刺激数据的缺乏反映了缺乏易于应用的单细胞刺激技术。我们提供了纳米刺激过程的详细描述，这是一种源自细胞旁标记技术的单细胞刺激方法。纳米刺激很容易应用，并且可以直接作用于麻醉和清醒动物的各种可识别的神经元。我们描述了记录方法和纳米刺激下电结构的参数。我们使用直流电阻为4-7 M ω的玻璃移液器。获得细胞旁结构需要移液管尖端和神经元之间的密切接触，并且与对>= 20 M ω值的阻力增加数倍有关。记录的动作电位（AP）振幅增长到>= 2 mV，神经元可以被纳安培范围内的电流激活，因此称为纳米刺激。虽然精确的AP定时尚未实现，但AP频率和AP数量可以参数化控制。我们证明纳米刺激也可以用来选择性地抑制可识别神经元的感觉反应。纳米刺激在生物物理上与电穿孔相似，基于这一假设，我们认为纳米刺激作用于移液管尖端正下方的微米区域的膜上，在那里膜孔是由高跨膜电压诱导的。有强有力的证据表明，纳米刺激选择性地激活单个神经元，所引起的效应是细胞特异性的。因此，纳米刺激在阐明单个神经元如何影响行为方面具有很大的潜力。
Houweling AR, Doron G, Voigt BC, Herfst LJ, Brecht M. Nanostimulation: manipulation of single neuron activity by juxtacellular current injection. J Neurophysiol 103: 1696-1704, 2010. First published December 2, 2009; doi: 10.1152/jn.00421.2009. In the mammalian brain, many thousands of single-neuron recording studies have been performed but less than 10 single-cell stimulation studies. This paucity of single-cell stimulation data reflects a lack of easily applicable single-cell stimulation techniques. We provide a detailed description of the procedures involved in nanostimulation, a single-cell stimulation method derived from the juxtacellular labeling technique. Nanostimulation is easy to apply and can be directed to a wide variety of identifiable neurons in anesthetized and awake animals. We describe the recording approach and the parameters of the electric configuration underlying nanostimulation. We use glass pipettes with a DC resistance of 4-7 M Omega. Obtaining the juxtacellular configuration requires a close contact between pipette tip and neuron and is associated with a several-fold increase in resistance to values >= 20 M Omega. The recorded action potential (AP) amplitude grows to >= 2 mV, and neurons can be activated with currents in the nanoampere range-hence the term nanostimulation. While exact AP timing has not been achieved, AP frequency and AP number can be parametrically controlled. We demonstrate that nanostimulation can also be used to selectively inhibit sensory responses in identifiable neurons. Nanostimulation is biophysically similar to electroporation, and based on this assumption, we argue that nanostimulation operates on membranes in the micrometer area directly below the pipette tip, where membrane pores are induced by high transmembrane voltage. There is strong evidence to suggest that nanostimulation selectively activates single neurons and that the evoked effects are cell-specific. Nanostimulation therefore holds great potential for elucidating how single neurons contribute to behavior.