Smooth muscle cell transplantation into myocardial scar tissue improves heart function
Smooth muscle cell transplantation into myocardial scar tissue improves heart function
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DOI：
10.1006/jmcc.1998.0882
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发表时间：
1999-03-01
影响因子：
5
通讯作者：
Mickle, DAG
中科院分区：
文献类型：
--
作者：
Li, RK;Jia, ZQ;Mickle, DAG
本研究旨在观察平滑肌细胞移植于冷冻坏死心肌瘢痕内的效果。成年大鼠左心室游离壁（LVFW）冷冻坏死。冷冻损伤后4周，移植培养的胎鼠胃平滑肌细胞（移植组，n=10）或培养液（对照组，n = 10）。假手术动物（n = 8）进行类似的手术，但没有冷冻坏死和移植。动物每日给予维持剂量的环孢菌素A（5 mg/kg）。在冷冻损伤后8周，使用Langendorff制备物评价动脉功能。对心肌瘢痕和移植细胞进行组织学评价。移植的平滑肌细胞存活并形成平滑肌细胞组织。如通过对心肌瘢痕内的平滑肌细胞肌动蛋白的免疫染色所评估的。在对照组的心脏中，在瘢痕中没有发现肌肉组织。血管生成（P
This study was designed to evaluate the effect of smooth muscle cell transplantation into myocardial ventricular scar formed by cryo-necrosis. The left ventricular free wall (LVFW) of adult rats was cryo-necrosed. At 4 weeks alter cryo-injury cultured fetal rat stomach smooth muscle cells (transplanted group, n=10) or culture medium (control, n = 10) were transplanted. Sham animals (n = 8) were similarly operated but without cryo-necrosis and transplantation. The animals were administered a daily maintenance dose of cyclosporin A (5 mg/kg). At 8 weeks after cryo-injury, he art function was evaluated using a Langendorff preparation. Myocardial scar and transplanted cells were assessed histologically. Transplanted smooth muscle cells survived and formed smooth muscle cell tissue. as assessed by immunostaining against smooth muscle cell actin, within the myocardial scar. In the control hearts no muscle tissue was found in the scar. Angiogenesis occur-ed (P