Effect of relaxin on in vitro fertilization of porcine oocytes.
Effect of relaxin on in vitro fertilization of porcine oocytes.
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松弛素对猪卵母细胞体外受精的影响。
DOI：
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发表时间：
2006
影响因子：
1.8
通讯作者：
H. Tsujii
中科院分区：
文献类型：
--
作者：
Young;A. G. Miah;Mayumi Yoshida;H. Sasada;K. Hamano;T. Kohsaka;H. Tsujii
猪松弛素是属于胰岛素超家族的肽类激素，具有多种生物学功能。本实验旨在研究松弛素对精子功能和猪卵母细胞体外受精（IVF）的影响。将猪精子洗涤、游走并在补充有0、20或50ng/ml猪松弛素的mTALP培养基中孵育1-4小时。通过观察精子向前运动的类型来确定活力，并通过应用三重染色技术评估顶体状态。从窦卵泡中吸出未成熟的卵母细胞并在 IVM 培养基（改良的 NCSU-37）中成熟。将成熟卵母细胞与精子在补充有0、5、10、15或20ng/ml松弛素的IVF培养基(mTALP)中共培养。精子-卵母细胞共孵育6小时后，将假定的受精卵在卵母细胞培养基NCSU-37中培养18小时，然后在乙酸地衣红染色后评估单精子、多精子和雄性原核形成率。在孵化1-4小时期间，松弛素改善了精子活力（P<0.05），并增加了顶体反应的活精子的百分比，但活力没有显着改善。显着地（P<0.05）与其他组相比，用 20 ng/ml 松弛素处理的精卵母细胞共培养组实现了最高的单精子受精百分比（31.7%）和最低的多精受精百分比（16.5%）。 20 ng/ml松弛素处理的精卵母细胞共培养组的雄性原核形成百分比显着高于其他组（P<0.05）。这些结果表明补充松弛素能够改善精子功能和体外猪卵母细胞的受精。
Porcine relaxin is a peptide hormone belonging to the insulin super family that has a variety of biological functions. The present experiment was designed to investigate the effects of relaxin on sperm function and on in vitro fertilization (IVF) of porcine oocytes. Porcine spermatozoa were washed, swum-up, and incubated for 1-4 h in mTALP medium supplemented with 0, 20 or 50 ng/ml porcine relaxin. Motility was determined by observing the type of forward movement of the spermatozoa, and acrosome status was evaluated by applying the triple staining technique. Immature oocytes were aspirated from antral follicles and matured in IVM medium (modified NCSU-37). Matured oocytes were co-cultured with spermatozoa in IVF medium (mTALP) supplemented with 0, 5, 10, 15 or 20 ng/ml relaxin. After 6 h of sperm-oocyte co-incubation, putative zygotes were cultured for 18 h in oocyte culture medium NCSU-37 and then assessed for the rates of monospermy, polyspermy, and male pronucleus formation after acetic orcein staining. Relaxin improved (P<0.05) sperm motility and increased the percentage of acrosome-reacted live spermatozoa during 1-4 h of incubation, although viability was not significantly improved. Significantly (P<0.05) the highest percentage of monospermic (31.7%) and lowest percentage of polyspermic (16.5%) fertilization was achieved from the sperm-oocyte co-culture group treated with 20 ng/ml relaxin as compared to other groups. The percentage of male pronucleus formation was significantly (P<0.05) greater in the 20 ng/ml relaxin-treated sperm-oocyte co-culture group than in the other groups. These results indicate that supplementation with relaxin is capable of improving sperm function and fertilization of porcine oocytes in vitro.
影响因子：
6.7
作者：
J. Colón;F. Ginsburg;J. Lessing;C. Schoenfeld;L. Goldsmith;R. Amelar;L. Dubin;G. Weiss
通讯作者：
J. Colón;F. Ginsburg;J. Lessing;C. Schoenfeld;L. Goldsmith;R. Amelar;L. Dubin;G. Weiss
松弛素对人类精子的影响。
DOI：
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发表时间：
1986
期刊：
The Journal of reproductive medicine
影响因子：
--
作者：
Lessing,JB;Brenner,SH;Colon,JM;Ginsburg,FW;Schoenfeld,C;Goldsmith,LT;Sarosi,P;Amelar,RD;Dubin,L;Weiss,G
通讯作者：
Weiss,G
人颗粒细胞培养物分泌的松弛素可预测体外受精-胚胎移植的成功。
DOI：
10.1093/humrep/14.2.338
发表时间：
1999
期刊：
Human reproduction (Oxford, England)
影响因子：
--
作者：
Stewart,DR;VandeVoort,CA
通讯作者：
VandeVoort,CA