The efficacy of an inhibin DNA vaccine delivered by attenuated Salmonella choleraesuis on follicular development and ovulation responses in crossbred buffaloes
The efficacy of an inhibin DNA vaccine delivered by attenuated Salmonella choleraesuis on follicular development and ovulation responses in crossbred buffaloes
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猪霍乱沙门氏菌减毒抑制素 DNA 疫苗对杂交水牛卵泡发育和排卵反应的功效
DOI：
10.1016/j.anireprosci.2016.07.004
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发表时间：
2016-09-01
影响因子：
2.2
通讯作者：
Yang, Liguo
中科院分区：
文献类型：
--
作者：
Liu, Qing;Han, Li;Yang, Liguo
本研究的目的是评估减毒猪霍乱沙门氏菌传递的Escherobin DNA疫苗对杂交水牛卵泡发育和排卵反应的效力。共158头杂交水牛分为4组，分别在第0天和第14天肌肉注射10 ml PBS中的1 x 10（10）（T1，n=41）、1 x 10（9）（T2，n=37）、1 x 10（8）（T3，n=37）或0（C，n=43）CFU/ml细菌递送的伊莱克斯宾疫苗。所有动物在第28天给予1000 IU PMSG，在第30天给予0.5 mg PGF（2 α），在第32天给予200 μ g GnRH。结果表明，T1组水牛的抗EscherichinIgG抗体滴度显著高于对照组（P= 10 mm），T1组排卵卵泡数显著高于C组（P
The aim of this study was to evaluate the efficacy of an inhibin DNA vaccine delivered by attenuated Salmonella choleraesuis on follicular development and ovulation responses in crossbred buffaloes. A total of 158 crossbred buffaloes divided into four groups and were intramuscularly injected with 1 x 10(10) (T1, n=41), 1 x 10(9) (T2, n=37), 1 x 10(8) (T3, n=37) or 0 (C, n=43) CFU/ml bacteria delivered inhibin vaccine in 10 ml PBS on day 0 and 14, respectively. All animals were administered with 1000 IU PMSG on day 28, 0.5 mg PGF(2 alpha), on day 30 and 200 mu g GnRH on day 32. The results showed buffaloes immunized with the bacteria delivered inhibin vaccine had significantly higher titers of anti-inhibin IgG antibody than control group (P= 10 mm) as well as ovulatory follicles in group T1 was significantly greater than group C (P