Heterologous amplification of microsatellite markers from colubroid snakes in European natricines (serpentes: natricinae).
Heterologous amplification of microsatellite markers from colubroid snakes in European natricines (serpentes: natricinae).
复制标题
欧洲水蛇（蛇类：natricinae）中蛇类蛇的微卫星标记的异源扩增。
作者：
A. Hille;I. Janssen;S. Menken;M. Schlegel;R. Thorpe
在跨物种扩增实验中，对为一系列蛇物种(新大陆水蛇、海蛇、鳄鱼)开发的18个微卫星基因座与欧洲水蛇(Natrix Natrix、N.Maura和N.tessellata)进行了比较。其中5个座位为多态(平均期望杂合度为0.749，平均样本量为47.8%)，3个座位为单态。其余的不能得到一致的分数或未能放大。对一组不同种类的八种眼镜蛇个体的进一步测试表明，18个基因座中的15个可以在这些物种中的至少一个物种中交叉扩增。我们的结论是，我们的结果显示了利用这些标记进行欧洲钠蛇类亲缘关系密切的欧洲钠蛇类的遗传变异的实验评估的前景，重点是N.Natrix。目前可用于蛇类的全套微卫星标记可能会显示出后续对更广泛范围的蛇类进行研究的额外潜力。
Eighteen microsatellite loci developed for a range of snake species (New World natricines, elapids, crotalids) were tested against European natricines (Natrix natrix, N. maura, and N. tessellata) in cross-species amplification experiments. Five loci were polymorphic (average expected heterozygosity 0.749 for a population of N. natrix in Amsterdam, mean sample size 47.8) and three loci were monomorphic. The remainder could not be consistently scored or failed to amplify. Further tests on single individuals of a diverse set of eight species of colubroid snakes showed that 15 of the 18 loci could be cross-amplified in at least one of these species. We conclude that our results show promise for the utilization of these markers for experimental assessments of genetic variation in the phylogenetically closely related group of European natricine snakes with emphasis on N. natrix. The full suite of microsatellite markers now available for snakes may show additional potential for subsequent investigation across a broader range of colubroid snakes.
小鹿罩鼠，小鹿突变以及红眼和红眼稀释基因的相互作用。
DOI：
10.1093/oxfordjournals.jhered.a109500
发表时间：
1981
期刊：
The Journal of heredity
影响因子：
--
作者：
LaVail,MM
通讯作者：
LaVail,MM
多个微卫星位点基于荧光的半自动基因分型与放射自显影技术的比较。
影响因子：
4.4
作者：
Schwengel,DA;Jedlicka,AE;Nanthakumar,EJ;Weber,JL;Levitt,RC
通讯作者：
Levitt,RC