GENERAL METHOD FOR ISOLATION OF HIGH MOLECULAR-WEIGHT DNA FROM EUKARYOTES
GENERAL METHOD FOR ISOLATION OF HIGH MOLECULAR-WEIGHT DNA FROM EUKARYOTES
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DOI：
10.1093/nar/3.9.2303
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发表时间：
1976-01-01
影响因子：
14.9
通讯作者：
STAFFORD, DW
中科院分区：
文献类型：
--
作者：
BLIN, N;STAFFORD, DW
A new method for isolation of high molecular weight DNA from eukaryotes is presented. This procedure allows preparation of DNA from a variety of tissues such as calf thymus or human placenta and from cells which were more difficult to lyse until now (e.g. Crypthecodinium cuhnii, a dinoflagellate). The DNA obtained in such a way has an average molecular weight of about 200 × 106d and contains very few, if any, single strand breaks.
