In Vivo 3-Dimensional Radiopharmaceutical-Excited Fluorescence Tomography
In Vivo 3-Dimensional Radiopharmaceutical-Excited Fluorescence Tomography
复制标题
体内 3 维放射性药物激发荧光断层扫描。
DOI：
10.2967/jnumed.116.180596
复制
发表时间：
2017-01-01
影响因子：
9.3
通讯作者：
Tian, Jie
中科院分区：
文献类型：
--
作者：
Hu, Zhenhua;Zhao, Mingxuan;Tian, Jie
切伦科夫发光成像可以使用高灵敏度电荷耦合器件相机对放射性药物进行成像。然而，放射性药物发出的切伦科夫发光较弱，在生物组织中的穿透深度较低，严重限制了切伦科夫发光成像的灵敏度和准确性。采用氧化铕纳米粒子进行三维放射药物激发荧光层析成像（REFT），增强了切伦科夫发光信号强度，提高了穿透深度，获得了更精确的放射药物三维分布。方法：体外检测EO纳米颗粒对F-18-FDG、(GaCl3)-Ga-68、Na(99)mTcO(4)等多种放射性药物的增强光信号。通过假体、人工源植入小鼠模型和肝癌小鼠实验，重建REFT放射药物的位置和三维分布，并与Cerenkov发光层析成像进行比较。结果：(GaCl3)-Ga-68和EO纳米粒子的光信号最强。体外幻影和植入小鼠研究表明，REFT显示了更准确的(GaCl3)-Ga-68的3D分布。在肝癌小鼠中，REFT比小动物PET能检出更多的肿瘤，获得比Cerenkov发光断层扫描更准确的三维分布信息。结论：EO纳米颗粒的REFT明显提高了放射性药物的定位精度，可以在体内精确定位肿瘤。
Cerenkov luminescence imaging can image radiopharmaceuticals using a high-sensitivity charge-coupled device camera. However, Cerenkov luminescence emitted from the radiopharmaceuticals is weak and has low penetration depth in biologic tissues, which severely limits the sensitivity and accuracy of Cerenkov luminescence imaging. This study presents 3-dimensional (3D) radiophamiaceutical-excited fluorescence tomography (REFT) using europium oxide (EO) nanoparticles, which enhances the Cerenkov luminescence signal intensity, improves the penetration depth, and obtains more accurate 3D distribution of radiopharmaceuticals. Methods: The enhanced optical signals of various radiopharmaceuticals (including F-18-FDG, (GaCl3)-Ga-68, Na(99)mTcO(4)) by EO nanoparticles were detected in vitro. The location and 3D distribution of the radiopharmaceuticals of REFT were then reconstructed and compared with those of Cerenkov luminescence tomography through the experiments with the phantom, artificial source-implanted mouse models, and mice bearing hepatocellular carcinomas. Results: The mixture of (GaCl3)-Ga-68 and EO nanoparticles possessed the strongest optical signals compared with the other mixtures. The in vitro phantom and implanted mouse studies showed that REFT revealed more accurate 3D distribution of (GaCl3)-Ga-68. REFT can detect more tumors than small-animal PET in hepatocellular carcinoma-bearing mice and achieved more accurate 3D distribution information than Cerenkov luminescence tomography. Conclusion: REFT with EO nanoparticles significantly improves accuracy of localization of radiopharmaceuticals and can precisely localize the tumor in vivo.