Effect of antibody and complement on permeability control in ascites tumor cells and erythrocytes.
Effect of antibody and complement on permeability control in ascites tumor cells and erythrocytes.
复制标题
DOI：
10.1084/jem.110.5.699
复制
发表时间：
1959-11-01
期刊：
影响因子：
--
通讯作者：
GOLDBERG B
中科院分区：
文献类型：
--
作者：
GREEN H;BARROW P;GOLDBERG B
兔抗体+补体改变小鼠Krebs腹水瘤细胞和红细胞的渗透性。当抗体+ C'作用于低蛋白培养基中的腹水肿瘤细胞时，细胞内K+以远大于正常渗漏速率的速率从细胞中损失。与此同时，细胞失去氨基酸和核糖核苷酸，并变得完全渗透介质中的Na+。当抗体+ C'在低蛋白质培养基中起作用时，细胞广泛溶胀并将其大部分大分子损失到培养基中（来自红细胞的血红蛋白、来自腹水肿瘤细胞的蛋白质和RNA）。如果抗体+ C'在含有足够浓度的蛋白质以平衡细胞的胶体渗透压的培养基中起作用，则肿胀被阻止;然后没有大分子从细胞中损失，但是K+的损失和Na+的进入没有改变，并且氨基酸和核糖核苷酸的损失仅受到轻微影响。因此，似乎抗体+ C'的作用是在动物细胞膜中产生功能性“孔”，其允许阳离子和小分子在细胞和培养基之间平衡。这导致细胞的渗透压增加和水的快速流入。细胞膜和它的“洞”因此被拉伸，允许大分子从细胞中逃逸。
Rabbit antibody + complement alters the permeability properties of mouse Krebs ascites tumor cells and erythrocytes. When antibody + C' acts on ascites tumor cells in a low protein medium, intracellular K+ is lost from the cells at a rate far greater than the normal leak rate. At the same time the cells lose amino acids and ribonucleotides and become fully permeable to the Na+ of the medium. When antibody + C' acts in a low protein medium, the cells swell extensively and lose most of their macromolecules to the medium (hemoglobin from erythrocytes, protein and RNA from the ascites tumor cells). If the antibody + C' acts in a medium containing protein in sufficient concentration to balance the colloid osmotic pressure of the cells, the swelling is prevented; no macromolecules are then lost from the cells, but the loss of K+ and entrance of Na+ are not altered, and the loss of amino acids and ribonucleotides is only slightly affected. It therefore appears that the action of antibody + C' is to produce functional "holes" in the animal cell membrane which permit the equilibration of cations and small molecules between cell and medium. This leads to an increase in the osmotic pressure of the cell and a rapid influx of water. The cell membrane and its "holes" are thereby stretched, permitting macromolecules to escape from the cell.