Evaluation of methanogenic strains and their ability to endure aeration and water stress
Evaluation of methanogenic strains and their ability to endure aeration and water stress
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DOI：
10.1007/s00284-007-9059-7
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发表时间：
2008-03-01
影响因子：
2.6
通讯作者：
Oyaizu, Hiroshi
中科院分区：
文献类型：
--
作者：
Liu, Chi-Te;Miyaki, Taro;Oyaizu, Hiroshi
在排水期间，水分胁迫和氧气都会对本土产甲烷菌造成损害。在本研究中，我们评估了7株产甲烷菌株(嗜铬甲烷短杆菌、甲酸甲烷杆菌、万尼氏甲烷球菌、洪氏甲烷螺杆菌、绿色甲烷假单胞菌、利氏甲烷假单胞菌和马氏甲烷八叠球菌)对长期暴露于空气/氮气和干燥的耐受性。我们发现，除利米科拉分枝杆菌和奥兰唐氏分枝杆菌外，这些产甲烷菌株在预干细胞中对干燥和氧气暴露的抵抗力比在浓缩液体培养中的菌株更强。在甲酸分枝杆菌的情况下，这种菌株的液体培养在与新鲜或无菌的土壤很好地混合时可以保持存活，但在没有土壤或琼脂泥浆的情况下培养则不能。这些结果表明，本地产甲烷菌定位于土壤隔间，以保护自己免受在含氧大气中逐渐干燥所造成的损害。
During periods of drainage, both water stress and oxygen can cause damage to indigenous methanogens. In the present study, we evaluated the tolerance of seven methanogenic strains (Methanobrevibacter arboriphilicus, Methanobacterium formicicum, Methanococcus vannielii, Methanospirillum hungatei, Methanoculleus olentangyi, Methanoplanus limicola, and Methanosarcina mazei) to long-term exposure to air/nitrogen and drying. We found that these methanogenic strains except for M. limicola and M. olentangyi in pre-dried cells offered more tenacious resistance to desiccation and oxygen exposure than those in enriched liquid cultures. In the case of M. formicicum, the liquid culture of this strain could remain viable when mixed well with fresh or sterile soil, but not when cultured without soil, or with agar slurry. These results suggest that indigenous methanogens localize within soil compartments to protect themselves from the damage caused by gradual drying under an oxic atmosphere.