RAM RETE TESTIS FLUID CONTAINS A PROTEIN (CLUSTERIN) WHICH INFLUENCES CELL-CELL INTERACTIONS INVITRO
RAM RETE TESTIS FLUID CONTAINS A PROTEIN (CLUSTERIN) WHICH INFLUENCES CELL-CELL INTERACTIONS INVITRO
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DOI：
10.1095/biolreprod28.5.1173
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发表时间：
1983-01-01
影响因子：
3.6
通讯作者：
BLASCHUK, O
中科院分区：
文献类型：
--
作者：
FRITZ, IB;BURDZY, K;BLASCHUK, O
Ram rete testis fluid elicited clustering of suspensions of Sertoli cells from testes of immature rats, TM-4 cells derived from mouse testis and erythrocytes from several species. Bioassay procedures and characteristics of the phenomenon are detailed. Concanavalin A and wheat germ agglutinin prevent aggregation elicited by rete testis fluid, and this inhibition is specifically prevented by .alpha.-methylmannoside and N-acetyl-glucosamine, respectively. Influences of rete testis fluid on cell aggregation are not dependent on exogenous calcium, but clustering is blocked by various metabolic inhibitors such as dinitrophenol. Rete testis fluid addition to mixed suspensions of erythrocytes and TM-4 cells is followed by separate aggregation of each cell type. Using aggregation of TM-4 cells suspended in simple medium at low density in rotation as a bioassay, fractions in rete testis fluid which retain activity were determined. A heat-stable, trypsin-sensitive protein, having an isoelectric point below pH 4.0, retains the capacity to aggregate cells. The possible functions of this protein, named clusterin, in cell interactions are discussed.
