Structural studies on chick embryonic hemoglobins.
Structural studies on chick embryonic hemoglobins.
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鸡胚胎血红蛋白的结构研究。
DOI：
10.1016/s0021-9258(19)42568-4
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发表时间：
1974
期刊：
影响因子：
--
通讯作者：
V. Ingram
中科院分区：
文献类型：
--
作者：
J. Brown;V. Ingram
雏鸡的红细胞生成涉及多种血红蛋白的合成。在胚胎晚期和成年鸡中发现的血红蛋白被称为血红蛋白A和D，在早期鸡胚中发现的血红蛋白被称为血红蛋白E、P和M。我们测定了胚胎血红蛋白E和P的肽链组成。在从5日龄胚胎的红细胞裂解物中分离血红蛋白E和P时，发现了一种先前未被怀疑的血红蛋白(M)，它似乎也存在于7日龄胚胎中。用兔抗血红蛋白A和D的抗血清，发现E和A的抗原性非常相似，并且M和D也是相关的A、D、E、P和M的氨乙基化球蛋白的肽图比较证实了这种关系。血红蛋白E似乎含有血红蛋白A的α链，而另一个(ε)链的肽图与成人血红蛋白A的β链有一些相似。对A球蛋白及其单链和E球蛋白及其单链的氨基酸分析证实，A和E的α链非常相似，而这两个血红蛋白的ε和β链不同。这两个血红蛋白的α链不同。π和π‘，以及一家类似β的连锁店ρ。ρ和ε链在它们的肽图上有许多共同的多肽。血红蛋白M包含在血红蛋白D中发现的αd链，而血红蛋白M的β样链类似于血红蛋白E的ε链。
Erythropoiesis in the chick involves the synthesis of multiple hemoglobins. The hemoglobins found in the late embryo and in the adult chicken are called hemoglobins A and D, and the hemoglobins found in the early chick embryo are called hemoglobins E, P, and M. We have determined the peptide chain composition of the embryonic hemoglobins E and P. During the chromatographic separation of hemoglobins E and P from the red cell lysate of a 5-day embryo, a previously unsuspected hemoglobin (M) was discovered which appears to be present also in 7-day embryos.Using rabbit antisera against hemoglobins A and D, it was found that hemoglobin E was very similar antigenically to A and that hemoglobins M and D were also related, although not as closely as hemoglobins E and A. Comparison of peptide maps of theS-aminoethylated globins of A, D, E, P, and M confirmed these relationships.Hemoglobin E appears to contain the αachain of hemoglobin A, while the peptide map of the other (ε) chain bears some resemblance to that of the β chain of adult hemoglobin A. An amino acid analysis of A globin and its individual chains and of E globin and its individual chains confirmed that the α chains of hemoglobins A and E are very similar, if not identical, while the ε and β chains of these two hemoglobins differ.Hemoglobin P is composed of two closely related α-like chains, π and π′, and a β-like chain, ρ. The ρ and the ε chain share many common peptides on their peptide maps.Hemoglobin M contains the αdchain found in hemoglobin D, while the β-like chain of hemoglobin M resembles the ε chain of hemoglobin E.