Establishment of an Efficient Genetic Transformation System of Cucumber and Transformation of Ginkgo Anti-fungal Peptide Gene
Establishment of an Efficient Genetic Transformation System of Cucumber and Transformation of Ginkgo Anti-fungal Peptide Gene
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发表时间：
2011
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通讯作者：
L. Jun
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作者：
L. Jun
建立了黄瓜快速再生体系和高效遗传转化体系，并对银杏抗真菌肽GK-2基因进行了遗传转化。结果表明，MS 6(MS+0.005 mg/L TDZ+0.2 mg/L IAA)培养基适合黄瓜愈伤组织的形成和不定芽的分化，7d内愈伤组织诱导率为100%，分化率为86.67%。获得了侵染时间为20min、共培养3d的最佳遗传转化体系和100 mg/L的卡那霉素抗性筛选浓度，转化阳性率为17.9%，转基因植株对枯萎病表现出较高的抗性。
A fast regeneration system and a highly efficient genetic transformation system of cucumber were established,and the transformation of Ginkgo anti-fungal peptide GK-2 gene was also done.The result showed that MS6(MS+0.005 mg/L TDZ+0.20 mg/L IAA)medium was suitable for cucumber callus formation and adventitious bud differentiation,which exhibited 100% callus rate and 86.67% differentiation rate within 7 days.Transferring Ginkgo anti-fungal peptide GK-2 gene into cucumber by Agrobacterium-mediated,we obtained the optimized genetic transformation system of 20 min infection time and 3 days co-culture as well as 100 mg/L kanamycin resistance screening concentration.Using this system,the positive rate was 17.9%,and transgenic plants exhibited a high resistance to Fusarium wilt.