CONVERGENCE OF ETS-RELATED AND NOTCH-RELATED STRUCTURAL MOTIFS IN A HETEROMERIC DNA-BINDING COMPLEX
CONVERGENCE OF ETS-RELATED AND NOTCH-RELATED STRUCTURAL MOTIFS IN A HETEROMERIC DNA-BINDING COMPLEX
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DOI：
10.1126/science.1876833
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发表时间：
1991-08-16
期刊：
影响因子：
56.9
通讯作者：
MCKNIGHT, SL
中科院分区：
文献类型：
--
作者：
THOMPSON, CC;BROWN, TA;MCKNIGHT, SL
异聚体DNA结合蛋白GABP的分析揭示了两个不同的肽序列基序的相互作用，通常与位于不同细胞区室的蛋白质相关。GABP的α-亚基含有与DNA结合蛋白的Ets家族相关的85个氨基酸的片段。GABP-alpha的ETS结构域促进与DNA的弱结合，并与相邻的37个氨基酸片段一起介导与GABP-beta的稳定相互作用。GABP的β-亚基包含存在于几种跨膜蛋白中的序列的四个不完全重复，包括果蝇Notch基因的产物。这些氨基末端重复的GABP-beta介导与GABP-alpha的稳定相互作用，当与GABP-alpha复合时，直接接触DNA。这些观察结果提供了一个独特的生化作用的33个氨基酸的重复序列的证据，并建议他们可以作为一个模块的产生特定的二聚化接口。
Analysis of the heteromeric DNA binding protein GABP has revealed the interaction of two distinct peptide sequence motifs normally associated with proteins located in different cellular compartments. The alpha-subunit of GABP contains an 85-amino acid segment related to the Ets family of DNA binding proteins. The ETS domain of GABP-alpha facilitates weak binding to DNA and, together with an adjacent segment of 37 amino acids, mediates stable interaction with GABP-beta. The beta-subunit of GABP contains four imperfect repeats of a sequence present in several transmembrane proteins including the product of the Notch gene of Drosophila melanogaster. These amino-terminal repeats of GABP-beta mediate stable interaction with GABP-alpha and, when complexed with GABP-alpha, directly contact DNA. These observations provide evidence for a distinct biochemical role for the 33-amino acid repeats, and suggest that they may serve as a module for the generation of specific dimerization interfaces.