cDNA cloning of nuclear localization signal binding protein NBP60, a rat homologue of lamin B receptor, and identification of binding sites of human lamin B receptor for nuclear localization signals and chromatin.
cDNA cloning of nuclear localization signal binding protein NBP60, a rat homologue of lamin B receptor, and identification of binding sites of human lamin B receptor for nuclear localization signals and chromatin.
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核定位信号结合蛋白 NBP60（核纤层蛋白 B 受体的大鼠同源物）的 cDNA 克隆，以及人核纤层蛋白 B 受体核定位信号和染色质结合位点的鉴定。
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
T. Horigome
中科院分区：
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作者：
S. Kawahire;M. Takeuchi;T. Gohshi;S. Sasagawa;M. Shimada;M. Takahashi;T. Abe;T. Ueda;R. Kuwano;A. Hikawa;T. Ichimura;S. Omata;T. Horigome
我们之前在大鼠肝核包膜中纯化并表征了核定位信号（NLS）结合蛋白 NBP60。在本研究中，我们克隆并测序了大鼠NBP60的cDNA，并预测了包含620个氨基酸的氨基酸序列。序列显示，NBP60 是核纤层蛋白 B 受体 (LBR) 的大鼠同源物，其氨基酸与人和鸡 LBR 的氨基酸分别有 79% 和 63% 相同。使用含有人 LBR 氨基末端结构域不同部分的三种融合蛋白，结果表明，包含氨基酸 1 至 89 的序列（其中含有富含 Ser-Arg 的区域（RS 区域））与核蓝蛋白结合，并且该结合被常见的 NLS 肽抑制。这些结果表明 LBR 的氨基末端结构域包含 NLS 结合位点。此外，显示包含氨基酸1至53的片段（不包含RS区域或预测的DNA结合位点）与非洲爪蟾精子染色质结合。
We previously purified and characterized a nuclear localization signal (NLS) binding protein, NBP60, in rat liver nuclear envelopes. In this study, we cloned and sequenced the cDNA of rat NBP60, and predicted an amino acid sequence comprising 620 amino acids. The sequence revealed that NBP60 is a rat homologue of lamin B receptor (LBR), and is 79 and 63% identical in amino acids to human and chicken LBR, respectively. Using three fusion proteins containing different parts of the amino-terminal domain of human LBR, it was shown that the stretch comprising amino acids 1 to 89, which contains a Ser-Arg rich region (RS region), binds to nucleoplasmin and that the binding was inhibited by a common NLS-peptide. These results suggested that the amino-terminal domain of LBR contains an NLS-binding site. Furthermore, it was shown that the stretch comprising amino acids 1 to 53, which does not contain the RS region or the predicted DNA-binding site, binds to Xenopus laevis sperm chromatin.