Studies of genomes of dairy bacteria Lactococcus lactis
Studies of genomes of dairy bacteria Lactococcus lactis
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DOI：
10.3166/sda.22.45-53
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发表时间：
2002-01-01
影响因子：
--
通讯作者：
Sorokin, A
中科院分区：
文献类型：
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作者：
Bolotin, A;Ehrlich, SD;Sorokin, A
乳酸乳球菌是奶酪发酵剂中最常见的细菌。据估计，人类每年消耗超过1018种细菌。从工业来源分离并从质粒中治愈的两个菌株IL 1403和MG 1363用于DNA克隆并研究该细菌的遗传学和分子生物学。这些菌株属于两个乳酸乳球菌菌株群，称为lactis和cremoris，不同的乳制品发酵产品的光谱。为L.我们对菌株IL 1403的全基因组进行测序，并对菌株MG 1363的染色体进行共线支架。共线支架包括有限数量的克隆的随机测序和两个基因组的共线区域的长距离PCR作图，其中一个基因组在较早时被完全测序。在通过PCR建立的共线性区域中，基因含量和顺序应该相同。MG1363基因组的共线支架与IL1403基因组具有85%的同一性，这使得容易获得来自该菌株的50%的基因。
Lactococcus lactis is the bacterium, which is most common in cheese starters. It is estimated that humans annually consume more than 1018 of these bacteria. Two strains, IL1403 and MG1363, isolated from industrial sources and cured from plasmids, are used for DNA cloning and to study genetics and molecular biology of this bacterium. These strains belong to two L, lactis strain clusters called lactis and cremoris, distinct by the spectra of dairy fermentation products. To create a basis for genomic studies of L. lactis we sequenced entire genome of the strain IL1403 and performed co-linear scaffolding of the chromosome of the strain MG1363. Co-linear scaffolding consists in random sequencing of a limited number of clones and Long Range PCR mapping of the co-linear regions of the two genomes, one of which was completely sequenced earlier. In the co-linear regions, established by PCR, gene content and order should be the same. The co-linear scaffolding of the MG1363 genome, which shares 85% identity with that of IL1403, allows easy access to 50% of the genes from this strain.