Concentration effects of three common fish anesthetics on Pacific hagfish (Eptatretus stoutii)
Concentration effects of three common fish anesthetics on Pacific hagfish (Eptatretus stoutii)
复制标题
三种常见鱼类麻醉剂对太平洋盲鳗（Eptatretus stoutii）的浓度影响
DOI：
10.1007/s10695-020-00761-4
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发表时间：
2020
影响因子：
2.9
通讯作者：
D. Fudge
中科院分区：
文献类型：
--
作者：
C. McCord;Emma Whiteley;Jessica Liang;Cathy Trejo;Rebecca Caputo;Estefania Itehua;Hina Hasan;Stephanie Hernandez;Kevin Jagnandan;D. Fudge
三种常见的鱼类麻醉剂（鱼油，2-苯氧乙醇，和三卡因甲磺酸盐）的效果进行了评估，在太平洋盲鳗（Eptatretus stoutii）。我们研究的首要目标是确定用于太平洋盲鳗常规实验室使用的最佳麻醉剂和浓度（即，诱导和恢复时间短且一致，并将对盲鳗的压力和安全风险降至最低）。我们的研究目的有四个方面：（1）确定太平洋八目鳗使用鱼油麻醉的麻醉阶段;（2）建立标准化的麻醉准备程序;（3）确定安全达到第V阶段麻醉的最佳麻醉剂和浓度;（4）研究重复暴露太平洋八目鳗麻醉的影响。实验浓度范围从50到400毫克/升，每种麻醉剂进行了测试，至少有三个太平洋盲鳗个人。我们发现以下内容：（1）太平洋盲鳗表现出与硬骨鱼相似的麻醉阶段;（2）鱼油与海水充分混合对麻醉诱导的一致性和时间有相当大的影响;（3）浓度和麻醉剂显著影响诱导和恢复时间，而体重对麻醉趋势没有影响;重复暴露于最佳浓度的鱼油或MS-222对诱导或恢复时间没有影响，而暴露次数显著影响使用2-PE时的诱导时间。由于诱导和恢复时间一致，意外过量的风险低，以及处理器和盲鳗的高安全裕度，我们建议将175 mg/L的鱼油作为太平洋盲鳗常规实验室使用的理想麻醉剂和浓度。
The efficacy of three common fish anesthetics (clove oil, 2-phenoxyethanol, and tricaine methanesulfonate) was evaluated in the Pacific hagfish (Eptatretus stoutii). The overarching aim of our study was to identify the best anesthetic and concentration for the purposes of routine laboratory use of Pacific hagfish (i.e., short and consistent induction and recovery times and minimized stress and safety risk to hagfish). The objectives of our study were fourfold: (1) identify anesthetic stages of Pacific hagfish using clove oil anesthesia; (2) establish standardized anesthesia preparation procedures; (3) determine the optimal anesthetic and concentration for safely achieving stage V anesthesia; and (4) investigate the effects of repeatedly exposing Pacific hagfish to anesthesia. Experimental concentrations, ranging from 50 to 400 mg/L, of each anesthetic were tested on at least three Pacific hagfish individuals. We found the following: (1) Pacific hagfish exhibited similar stages of anesthesia to those described for bony fishes; (2) sufficient mixing of clove oil with seawater had a considerable effect on the consistency and timing of anesthetic induction; (3) concentration and anesthetic significantly impacted induction and recovery timing, whereas body mass had no impact on anesthetic trends; and (4) repeatedly exposing Pacific hagfish to optimal concentrations of clove oil or MS-222 had no effect on induction or recovery timing, whereas exposure number significantly impacted induction timing when using 2-PE. Due to consistent induction and recovery times, low risk of accidental overdose, and high safety margins for both handler and hagfish, we recommend 175 mg/L of clove oil as the ideal anesthetic and concentration for the routine laboratory use of Pacific hagfish.