Gene expression demonstrates an immunological capacity of the human endolymphatic sac
Gene expression demonstrates an immunological capacity of the human endolymphatic sac
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DOI：
10.1002/lary.25242
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发表时间：
2015-08-01
期刊：
影响因子：
2.6
通讯作者：
Caye-Thomasen, Per
中科院分区：
文献类型：
--
作者：
Moller, Martin Nue;Kirkeby, Svend;Caye-Thomasen, Per
目的/假设本研究的目的是探索、证明和描述与先天免疫系统相关的基因在内淋巴囊中的表达。据推测，内淋巴囊具有显着的免疫功能，在人类innerear.Study DesignDNA微阵列和免疫组化被用于分析新鲜的人类内淋巴囊组织samples.MethodsTwelve的组织样本，从人类内淋巴囊获得在经尿道手术前庭神经鞘瘤。采用基因芯片技术，以邻近硬脑膜为对照，研究组织样本基因表达。先天免疫系统的特异性基因的表达进行了测定，并通过免疫组化验证选定的关键分子的结果。ResultsA先天免疫系统的表达基因的全面概述。细胞和体液的先天免疫系统的多个关键要素的表达，包括Toll样受体4和7，以及β-防御素和lactoferrin.ConclusionsThe目前的数据提供了第一个直接的证据，人类内淋巴囊的免疫能力。在分子水平上，内淋巴囊能够识别和处理抗原以启动免疫应答。此外，对入侵病原体直接有毒的有效分子由囊上皮表达。这一证据强烈支持内淋巴囊是内耳的一个重要的免疫实体。喉镜，125：E269-E275，2015
Objectives/HypothesisThe purpose of the present study is to explore, demonstrate, and describe the expression of genes related to the innate immune system in the human endolymphatic sac. It is hypothesized that the endolymphatic sac has a significant immunological function in the human inner ear.Study DesignDNA microarrays and immunohistochemistry were used for analyses of fresh human endolymphatic-sac tissue samples.MethodsTwelve tissue samples from the human endolymphatic sac were obtained during translabyrinthine surgery for vestibular schwannoma. Microarray technology was used to investigate tissue sample gene expression using adjacent dura mater as control. The expression of genes specific for the innate immune system was determined and results for selected key molecules verified by immunohistochemistry.ResultsA comprehensive overview of expressed genes of the innate immune system was obtained. Multiple key elements of both the cellular and humoral innate immune system were expressed, including Toll-like receptors 4 and 7, as well as beta-defensin and lactoferrin.ConclusionsThe present data provides the first direct evidence of an immunological capacity of the human endolymphatic sac. At the molecular level, the endolymphatic sac is capable of antigen recognition and processing for initiation of an immune response. In addition, potent molecules directly toxic to invading pathogens are expressed by the sac epithelium. This evidence strongly supports the endolymphatic sac as a significant immunological entity of the inner ear.Level of EvidenceN/A. Laryngoscope, 125:E269-E275, 2015