Cutaneous applied nano-ZnO reduce the ability of hair follicle stem cells to differentiate
Cutaneous applied nano-ZnO reduce the ability of hair follicle stem cells to differentiate
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皮肤应用纳米氧化锌降低毛囊干细胞的分化能力
DOI：
10.1080/17435390.2017.1310947
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发表时间：
2017-01-01
期刊：
影响因子：
5
通讯作者：
Shen, Wei
中科院分区：
文献类型：
--
作者：
Ge, Wei;Zhao, Yong;Shen, Wei
摘要在过去的十年中，金属氧化物纳米颗粒渗透皮肤的能力引起了人们极大的兴趣，这是由于对含有物理抗紫外线金属颗粒（如纳米氧化锌（nZnO））的局部应用防晒霜的安全性的担忧。以往的研究表明，金属氧化物纳米粒子积累在皮肤皱纹和毛囊局部应用后，而鲜为人知的是，这些纳米粒子对皮肤稳态的后果。目前的研究测试了nZnO（0.5 mg/天小鼠）对毛囊生理学的影响。局部应用凡士林含有nZnO，散装ZnO（bZnO），或离子化锌到新生小鼠触须垫超过7天的一段时间，显示nZnO积累在毛囊内，这诱导毛囊干细胞（HFSCs）的凋亡。体外研究也表明nZnO暴露可引起HFSCs明显的DNA损伤和诱导凋亡。此外，发现nZnO暴露干扰与HFSC凋亡、细胞通讯和分化相关的基因。HFSCs移植实验表明HFSCs分化潜能降低。这项调查表明，局部应用的ZnO纳米粒子对皮肤稳态的潜在风险。
Abstract The ability of metal oxide nanoparticles to penetrate the skin has aroused a great deal of interest during the past decade due to concerns over the safety of topically applied sunscreens that contain physical UV-resistant metal particles, such as nano-Zinc oxide (nZnO). Previous studies demonstrate that metal oxide nanoparticles accumulate in skin furrows and hair follicles following topical application while little is known about the consequence of these nanoparticles on skin homeostasis. The current investigation tested the effects of nZnO (0.5 mg/day mouse) on hair follicle physiology. Topical application of Vaseline containing nZnO, bulk ZnO (bZnO), or ionized Zn to newborn mice vibrissa pad over a period of 7 consecutive days revealed that nZnO accumulated within hair follicles, and this induced the apoptosis of hair follicle stem cells (HFSCs). In vitro studies also indicated that nZnO exposure caused obvious DNA damage and induced apoptosis in HFSCs. Furthermore, it was found that nZnO exposure perturbed genes associated with HFSC apoptosis, cell communication, and differentiation. HFSCs transplantation assay demonstrated that the potential of HFSCs to differentiate was reduced. This investigation indicates a potential risk of topically applied ZnO nanoparticles on skin homeostasis.