Somatic Embryogenesis and Plant Regeneration from Cotyledon and Hypocotyl Explants of Fagopyrum esculentum Moench lpls Mutant
Somatic Embryogenesis and Plant Regeneration from Cotyledon and Hypocotyl Explants of Fagopyrum esculentum Moench lpls Mutant
复制标题
苦荞 Moench lpls 突变体子叶和下胚轴外植体的体细胞胚胎发生和植物再生
DOI：
10.3390/agronomy9110768
复制
发表时间：
2019-11-01
期刊：
影响因子：
3.7
通讯作者：
Liu, Zhi-Xiong
中科院分区：
文献类型：
--
作者：
Fei, Yue;Wang, Lan-Xiang;Liu, Zhi-Xiong
荞麦（Fagopyrum esculentum，桑科）是一种具有治疗益处的一年生假谷类作物。然而，荞麦的二型花和异型自交不亲和性是其遗传改良的主要原因，其遗传改良的成效有限。在此，我们建立了一个有效的间接体细胞胚胎发生和植株再生的方法。蔬菜首先，下胚轴外植体在MS +2.0 mgL（-1）2，4-D +1.5 mgL（-1）6-BA的培养基上培养30 d，将愈伤组织转移到附加1.0 mgL（-1）6-BA和0.5 mgL（-1）6-BA的新鲜MS培养基上，可获得黄白色的脆性胚性愈伤组织。噻苯隆（TDZ）每隔40-60天继代2 - 3次。胚性愈伤组织在MS +6-BA 1.0 mgL（-1）+TDZ 0.5 mgL（-1）+15%马铃薯泥的培养基上继代培养20 d后，可产生体细胞胚。最后，将成熟的体细胞胚转移到不含生长调节剂的MS基本培养基上培养40天，获得了最高的平均体细胞胚植株百分率（75.75%）。本研究结果为苜蓿下胚轴外植体的再生和SE提供了一个有效的途径。蔬菜
Buckwheat (Fagopyrum esculentum, Family Polygonaceae) is an annual pseudo-cereal crop with healing benefits. However, the genetic improvement of common buckwheat has achieved only limited success, mainly due to buckwheat's dimorphic flowers and heteromorphic self-incompatibility. Here, we develop a useful protocol for indirect somatic embryogenesis and subsequent plant regeneration from hypocotyl explants of F. esculentum. Firstly, the initial calli of hypocotyl explants were induced on Murashige and Skoog (MS) basal medium containing 2.0 mgL(-1) 2,4-D and 1.5 mgL(-1) 6-BA for 30 days culture, and then the yellowish white friable embryogenic calli were developed when the initial calli were transferred to fresh MS basal medium supplemented with 1.0 mgL(-1) 6-BA and 0.5 mgL(-1) thidiazuron (TDZ)two to three times subculture at 40-60 days intervals. Subsequently, the somatic embryos were able to germinate from embryogenic callus sub-cultured on MS basal medium containing 1.0 mgL(-1) 6-BA and 0.5 mgL(-1) TDZ with 15% potato puree for 20 days subculture. Finally, maximum mean percentage (75.75%) of somatic embryo-derived plants were obtained when the mature somatic embryos were transferred to MS basal medium without growth regulators for 40 days culture. Our result provides a useful protocol for plant regeneration and SE from hypocotyl explants of F. esculentum.