Human Adipose Tissue in Culture - VII. The Long-Term Effect of Growth Hormone
Human Adipose Tissue in Culture - VII. The Long-Term Effect of Growth Hormone
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培养中的人类脂肪组织 - VII。
DOI：
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发表时间：
1977
期刊：
影响因子：
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通讯作者：
U. Smith
中科院分区：
文献类型：
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作者：
G. Nyberg;U. Smith
将人脂肪组织活检标本在体外培养一周，加入或不加入不同浓度的人生长激素（GH）。预孵育后，将其在体外孵育2小时，类似于通常用于短期实验的技术。在基础条件下以及在胰岛素或去甲肾上腺素存在下测量甘油的释放和葡萄糖掺入甘油三酯。基础葡萄糖掺入率的损害后，发现培养期与生长激素，但没有伴随的影响脂解。胰岛素对葡萄糖掺入的影响与对照组相同，但在胰岛素存在下代谢率甚至仍低于对照组。去甲肾上腺素和胰岛素对脂肪分解的影响与对照组相似。从本研究中得出结论，可以检测到GH对人脂肪组织的直接“致糖尿病”作用。在进行孵育的7天内，这种效应连续发展。这是由于葡萄糖掺入的基础速率降低，而不是对胰岛素作用的直接抵抗，也与脂解增加无关。
Biopsies of human adipose tissue were cultured for one week in vitro with or without different concentrations of human growth hormone (GH). After preincubation they were incubated in vitro for 2 hours analogous to the technique generally used for short-term experiments. The release of glycerol and incorporation of glucose into triglycerides were measured under basal conditions as well as in the presence of insulin or noradrenaline. An impairment in the basal glucose incorporation rate was found after the culture period with GH, but there was no concomitant effect on lipolysis. The effect of insulin on glucose incorporation was stimulating to the same extent as in the controls but the metabolis rate was even in the presence of insulin still below that of the controls. The effects of noradrenaline and of insulin on lipolysis were similar to those in the control group. It is concluded from the present study that a direct "diabetogenic" effect of GH on human adipose tissue can be detected. This effect developed successively over the seven days that the incubations were performed. It was due to a diminished basal rate of glucose incorporation and not to a direct resistance to the action of insulin and it was not related to increased lipolysis.