Time course of female-to-male sex reversal in 38,XX fetal and postnatal pigs
Time course of female-to-male sex reversal in 38,XX fetal and postnatal pigs
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DOI：
10.1002/dvdy.1194
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发表时间：
2001-11-01
影响因子：
2.5
通讯作者：
Cotinot, C
中科院分区：
文献类型：
--
作者：
Pailhoux, E;Parma, P;Cotinot, C
为了了解猪中间性的病因，我们彻底分析了38，XX（SRY阴性）雌性转雄性动物在不同发育阶段的性腺：在胎儿期[产后50和70天（dpc）]，刚出生后[产后35天（dpp）]和成年期。对于研究的每只动物，我们对一个性腺进行平行的组织学和超微结构分析，对另一个性腺进行RT-PCR分析，以确定性调节基因的表达谱：SOX 9，3 β-HSD，P450芳香化酶，AAM，FOXL 2和Wnt 4。光镜和电镜检查显示XX性反转性腺中有睾丸索分化，但发育不全。虽然睾丸索在胎儿时期含有生殖细胞，但生殖细胞在出生后几周内就通过凋亡而全部死亡。超微结构正常的Leydig细胞也分化，但后来，并封闭轮状残留机构。在胎儿阶段，早在出生后50天，双性人性腺中研究的六个基因中的三个就呈现出修饰的表达谱，对应于SOX 9表达的升高以及AMH和P450芳香酶基因转录的开始。除了参与睾丸途径的基因外，相同的性腺表达FOXL 2，一种卵巢特异性因子。真两性人的卵巢不能保证正常的卵泡发生，出生后卵巢特异性基因表达谱异常。这些结果表明，在这种病理作用的基因在性腺发育过程中非常早期，并影响卵巢分化途径与卵巢生殖细胞耗竭通过转分化成睾丸结构的可变表达。(C)2001 Wiley-Liss，Inc.
In an attempt to understand the etiology of intersexuality in pigs, we thoroughly analyzed the gonads of 38,XX (SRY negative) female to male sex-reversed animals at different developmental stages: during fetal life [50 and 70 days postcoitum (dpc)], just after birth [35 days postpartum (dpp)] and during adulthood. For each animal studied, we performed parallel histological and ultrastructural analyses on one gonad and RT-PCR analysis on the other gonad in order to define the expression profiles of sexually regulated genes: SOX9, 3 beta -HSD, P450 aromatase, AAM, FOXL2, and Wnt4. Light and electron microscopic examination showed that testicular cords differentiated in XX sex-reversed gonads but were hypoplastic. Although the testicular cords contained gonia at the fetal stages, the germ cells had all died through apoptosis within a few weeks after birth. Ultrastructurally normal Leydig cells also differentiated, but later, and enclosed whorl-like residual bodies. At the fetal stages, three of the six genes studied in the intersex gonads presented, as early as 50 dpe, a modified expression profile corresponding to an elevated expression of SOX9 and the beginning of AMH and P450 aromatase gene transcription. In addition to genes involved in the testicular pathway, the same gonads expressed FOXL2, an ovarian-specific factor. The ovaries of true hermaphrodites were ineffective in ensuring correct folliculogenesis and presented abnormal expression profiles of ovarian specific genes after birth. These results indicate that the genes involved in this pathology act very early during gonadogenesis and affect the ovary-differentiating pathway with variable expressivity from ovarian germ cell depletion through to trans-differentiation into testicular structures. (C) 2001 Wiley-Liss, Inc.