Biological activity of purified simian virus 40 T antigen proteins.
Biological activity of purified simian virus 40 T antigen proteins.
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纯化的猿猴病毒 40 T 抗原蛋白的生物活性。
DOI：
10.1073/pnas.75.3.1279
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发表时间：
1978
影响因子：
11.1
通讯作者：
A. Graessmann
中科院分区：
文献类型：
--
作者：
R. Tjian;G. Fey;A. Graessmann
从腺病毒2/SV40杂交体AD2+D2和AD2+ND1 DP2感染的细胞以及由SV40转化的人细胞系(SV80)中分离到与猴病毒40(SV40)T抗原相关的蛋白。SV80和AD2+D2的96,000和107,000道尔顿蛋白注入培养细胞的细胞质后，迅速聚集在细胞核中，在那里它们保持抗原性反应至少20小时，并在静止的细胞中触发DNA合成。相比之下，由AD2+ND1 DP2编码的23,000道尔顿蛋白质不刺激细胞DNA合成。然而，这三种纯化的蛋白都能够为腺病毒2在猴细胞中的生长提供辅助功能。因此，纯化的SV40T抗原和与其共享序列的蛋白质保持了至少两种与SV40A基因产物相关的功能。
Proteins related to simian virus 40 (SV40) T antigen uere isolated from cells infected with adenovirus 2/SV40 hybrids Ad2+D2 and Ad2+ND1 dp2 as well as from a line of human cells (SV80) transformed by SV40. The 96,000- and 107,000-dalton proteins of SV80 and Ad2+D2, after injection into the cytoplasm of cultured cells, rapidly accumulate in the nuclei, where they remain antigenically reactive for at least 20 hr and trigger DNA synthesis in quiescent cells. By contrast, the 23,000-dalton protein coded by Ad2+ND1 dp2 does not stimulate cellular DNA synthesis. However, all three purified proteins are able to provide helper function for the growth of adenovirus 2 in monkey cells. Thus, purified SV40 T antigen and proteins that share sequences with it retain the ability to carry out at least two functions associated with the product of the A gene of SV40.