Characterization of RACK1 function in Drosophila development
Characterization of RACK1 function in Drosophila development
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DOI：
10.1002/dvdy.21217
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发表时间：
2007-08-01
影响因子：
2.5
通讯作者：
Beckerle, Mary C.
中科院分区：
文献类型：
--
作者：
Kadrmas, Julie L.;Smith, Mark A.;Beckerle, Mary C.
活化C激酶1受体（Receptor for Activated C Kinase 1，RACK 1）是一种细胞质分子支架蛋白。据报道，参与关键信号传导途径的许多不同的蛋白质结合伴侣与RACK 1结合，表明RACK 1在信号整合中的作用。然而，由于RACK1在完整生物体中的功能丧失表型尚未报道，我们目前对RACK1的理解是有限的。使用果蝇，我们表明，RACK 1在所有发育阶段和许多组织中表达，在卵巢中有特定的富集。通过表征RACK 1突变体的等位基因系列，我们证明了RACK 1在果蝇发育的多个步骤中是必不可少的，
Receptor for Activated C Kinase 1 (RACK1) is a cytoplasmic molecular scaffolding protein. Many diverse protein-binding partners involved in key signaling pathways are reported to bind to RACK1, suggesting a role for RACK1 in signal integration. However, because loss-of-function phenotypes for RACK1 in an intact organism have not yet been reported, our current understanding of RACK1 is limited. Using Drosophila melanogaster, we show that RACK1 is expressed at all developmental stages and in many tissues, with specific enrichment in the ovary. By characterizing an allelic series of RACK1 mutants, we demonstrate that RACK1 is essential at multiple steps of Drosophila development,