Adaptional evolution of trichome in Caragana korshinskii to natural drought stress on the Loess Plateau, China.
Adaptional evolution of trichome in Caragana korshinskii to natural drought stress on the Loess Plateau, China.
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黄土高原柠条毛状体对自然干旱胁迫的适应进化
DOI：
10.1002/ece3.2157
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发表时间：
2016-06
影响因子：
2.6
通讯作者：
Gong C
中科院分区：
文献类型：
--
作者：
Ning P;Wang J;Zhou Y;Gao L;Wang J;Gong C
摘要柠条是黄土高原干旱生态系统的重要改良植物，但其作用机制尚不清楚，尤其是植物对干旱胁迫的耐受能力。水分是黄土高原植物生长的最大限制因子。已知毛状体通过降低蒸腾速率在减少水分损失方面发挥有效作用，因此在本研究中，我们专注于毛状体相关的生态适应基因表达。在低降水量条件下生长的柠条为了探讨毛状体对干旱的响应，我们沿着黄土高原纬度梯度从湿到干选择了两个实验地点进行观察。利用扫描电镜观察了降水减少条件下毛状体变大变密的微观现象;然后利用从头转录组和定量PCR探索和验证了C.红松毛状体。结果显示，GIS 2、TTG 1和GL 2上调（作为毛状体发育的关键正调控基因），而CPC下调（负调控基因）。综上所述，我们的数据表明，赤霉素和细胞分裂素信号通路的下游基因，以及几个细胞骨架相关基因，有助于调节毛状体的发育，以提高蒸腾抗性能力，提高对干旱胁迫的抗性。Korshinskii。
Abstract Caragana korshinskii is commonly employed to improve drought ecosystems on the Loess Plateau, although the molecular mechanism at work is poorly understood, particularly in terms of the plant's ability to tolerate drought stress. Water is the most severe limiting factor for plant growth on the Loess Plateau. The trichome is known to play an efficient role in reducing water loss through decreasing the rate of transpiration, so in this study, we focused on the trichome‐related gene expression of ecological adaptation in C. korshinskii under low precipitation conditions. In order to explore the responses of trichomes to drought, we selected two experimental sites from wet to dry along the Loess Plateau latitude gradient for observation. Micro‐phenomena through which trichomes grew denser and larger under reduced precipitation were observed using a scanning electron microscope; de novo transcriptomes and quantitative PCR were then used to explore and verify gene expression patterns of C. korshinskii trichomes. Results showed that GIS2,TTG1, and GL2 were upregulated (as key positive‐regulated genes on trichome development), while CPC was downregulated (negative‐regulated gene). Taken together, our data indicate that downstream genes of gibberellin and cytokinin signaling pathways, alongside several cytoskeleton‐related genes, contribute to modulating trichome development to enhance transpiration resistance ability and increase the resistance to drought stress in C. korshinskii.
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影响因子：
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作者：
Hauser MT
通讯作者：
Hauser MT
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作者：
Gibbons, FD;Roth, FP
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