ELECTRON-PARAMAGNETIC RESONANCE STUDIES ON HEPATIC MICROSOMAL CYTOCHROME-P-450 FROM A MARINE TELEOST FISH
ELECTRON-PARAMAGNETIC RESONANCE STUDIES ON HEPATIC MICROSOMAL CYTOCHROME-P-450 FROM A MARINE TELEOST FISH
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DOI：
10.1016/0024-3205(77)90043-1
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发表时间：
1977-01-01
期刊：
影响因子：
6.1
通讯作者：
BLUMBERG, WE
中科院分区：
文献类型：
--
作者：
CHEVION, M;STEGEMAN, JJ;BLUMBERG, WE
鱼类肝微粒体细胞色素P-450（Stenotomus versicolor）未处理或用3-甲基胆蒽、5，6-苯并异丁酮或三卡因甲磺酸盐处理的微粒体，当还原并连接到CO时，在450 nm处显示最大吸收。产生类似于来自各种其它来源的细胞色素P-450的晶体场参数。用3-甲基胆蒽或5，6-苯并吡喃酮处理导致芳烃（苯并[a]芘）羟化酶活性水平升高，但CO连接的细胞色素P-450的水平或光学性质没有明显变化。甲磺酸三卡因是一种常见的鱼类麻醉剂，可导致鱼类细胞色素P-450水平下降。
Hepatic microsomal cytochrome P-450 from fish (Stenotomus versicolor), untreated or treated with 3-methylcholanthrene, 5,6-benzoflavone or tricaine methanesulfonate, exhibited an absorption maximum at 450 nm when reduced and ligated to CO. Microsomes from all groups exhibited EPR spectra with g values near 2.4, 2.24 and 1.9, yielding crystal field parameters similar to those for cytochrome P-450 from a variety of other sources. Treatment with 3-methylcholanthrene or 5,6-benzoflavone resulted in elevated levels of aryl hydrocarbon (benzo[a]pyrene) hydroxylase activity yet produced no apparent change in the levels or optical properties of CO-ligated cytochrome P-450. Tricaine methanesulfonate, a common fish anaesthetic, caused a decrease in the levels of fish cytochrome P-450.