Imbalance of the interleukin 1 system in colonic mucosa - association with intestinal inflammation and interleukin 1 receptor agonist genotype 2
Imbalance of the interleukin 1 system in colonic mucosa - association with intestinal inflammation and interleukin 1 receptor agonist genotype 2
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DOI：
10.1136/gut.41.5.651
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发表时间：
1997-11-01
期刊：
影响因子：
24.5
通讯作者：
Gross, V
中科院分区：
文献类型：
--
作者：
Andus, T;Daig, R;Gross, V
背景-白细胞介素1（IL-1）α和β是在炎症中起关键作用的强效细胞因子。目的：研究粘膜炎症和IL-1ra基因型对IL-1ra：IL-1平衡的影响。方法：采用酶联免疫吸附试验（ELISA）检测60例克罗恩病（CD）、34例溃疡性结肠炎（UC）、15例炎症对照和103例非炎症对照的炎症和非炎症结肠活检标本中的IL-1 α、IL-1 β和IL-1ra。结果IL-1 α和IL-1 β在炎症性肠病（IBD）患者炎症黏膜中明显升高，IL-1 α和IL-1 β在IBD患者炎症黏膜中明显升高。（CD：53.5（22.4）和409.9（118.7）pg/mg蛋白; UC：分别为18.9（6.8）和214.5（78.2）pg/mg）和非IBD患者（分别为19.2（7.4）和281.4（121.0）pg/mg; p
Background-Interleukin 1 (IL-1) alpha and beta are potent cytokines which play key roles in inflammation. They are controlled by IL-1 receptor antagonist (IL-1ra).Aims-To investigate the influence of mucosal inflammation and IL-1ra genotype on the IL-1ra:IL-1 balance.Patients and methods-IL-1 alpha, IL-1 beta, and IL-1ra were measured by enzyme Linked immunosorbent assay (ELISA) in biopsy specimens taken from inflamed and non-inflamed colon of 60 patients with Crohn's disease (CD), 34 with ulcerative colitis (UC), 15 inflammatory controls, and 103 non-inflamed controls. IL-1ra genotype was determined by polymerase chain reaction and gel electrophoresis.Results-IL-1 alpha and IL-1 beta were significantly increased in inflamed mucosa in inflammatory bowel disease (IBD) (CD: 53.5 (22.4) and 409.9 (118.7) pg/mg protein, respectively; UC: 18.9 (6.8) and 214.5 (78.2) pg/mg, respectively) and non-IBD patients (19.2 (7.4) and 281.4 (121.0) pg/mg, respectively; p