Geranylated carbazole alkaloids with potential neuroprotective activities from the stems and leaves of Clausena lansium
Geranylated carbazole alkaloids with potential neuroprotective activities from the stems and leaves of Clausena lansium
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来自黄皮茎和叶的具有潜在神经保护活性的香叶基咔唑生物碱
DOI：
10.1016/j.bioorg.2019.103278
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发表时间：
2019
影响因子：
5.1
通讯作者：
Fu Yan-Hui
中科院分区：
文献类型：
--
作者：
Liu Yan-Ping;Guo Jia-Ming;Wang Xiao-Ping;Liu Yun-Yao;Zhang Wei;Wang Ting;Qiang Lei;Fu Yan-Hui
黄皮(皱眉头。)龙骨是一种具有重要经济价值的常绿小乔木或灌木，属芸香科龙骨属。兰属植物原产于中国南方，目前在亚热带和热带地区广泛种植，不仅因其果实的营养价值和药理作用，而且作为药用和观赏植物。在本研究中，系统地研究了紫菜茎叶的植物化学成分。lansium引起了两种新的香叶酰化咔唑生物碱的分离和鉴定，clauselansiumines A(1)和B(2)，以及10种已知的香叶酰化咔唑生物碱（3-12）。这些分离的香叶酰化咔唑生物碱（1-12）的化学结构是基于综合光谱数据分析明确确定的。在体外测试了这些分离的香叶酰化咔唑生物碱对6-羟多巴胺诱导的人神经母细胞瘤SH-SY5Y细胞死亡的神经保护作用。化合物1 - 12具有显著的神经保护作用，其ec50值为0.48 ± 0.04 ~ 12.36 ± 0.16 μM。这些研究结果表明，分离纯化的香叶酰化咔唑类生物碱具有显著的神经保护作用。镧可能对发现治疗和预防帕金森氏症的新药物非常重要。
Clausena lansium(Lour.) Skeels is an evergreen small tree or shrub with great economic value, which belongs to the genusClausenaof the Rutaceae family.C. lansiumis indigenous to Southern China, while currently widely cultivated in subtropical and tropical regions not only for the nutritional value and pharmacological uses of its fruits but also as a medicinal and ornamental plant. In this study, a systematic phytochemical study on the stems and leaves ofC. lansiumcaused the separation and identification of two new geranylated carbazole alkaloids, clauselansiumines A (1) and B (2), as well as 10 known geranylated carbazole alkaloids (3–12). The chemical structures of these isolated geranylated carbazole alkaloids (1–12) were unambiguously determined based on comprehensive spectral data analyses. All these isolated geranylated carbazole alkaloids were tested for their neuroprotective effects against 6-hydroxydopamine induced cell death in human neuroblastoma SH-SY5Y cellsin vitro. Compounds1–12displayed remarkable neuroprotective effects holding the EC50values ranging from 0.48 ± 0.04 to 12.36 ± 0.16 μM. These research results disclosed that the separation and purification of these geranylated carbazole alkaloids possessing remarkable neuroprotective effects separated fromC. lansiumcould be extremely important to the discovery of new agents for the treatment and prevention for Parkinson's disease.