MITOCHONDRIAL-DNA (MTDNA) - RELATEDNESS AMONG FORMAE-SPECIALES OF FUSARIUM-OXYSPORUM IN THE CUCURBITACEAE
MITOCHONDRIAL-DNA (MTDNA) - RELATEDNESS AMONG FORMAE-SPECIALES OF FUSARIUM-OXYSPORUM IN THE CUCURBITACEAE
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DOI：
10.1094/phyto-83-91
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发表时间：
1993-01-01
期刊：
影响因子：
3.2
通讯作者：
MAGILL, CW
中科院分区：
文献类型：
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作者：
KIM, DH;MARTYN, RD;MAGILL, CW
通过线粒体 DNA (mtDNA) 的限制性片段长度多态性 (RFLP) 分析，检查了 39 个尖孢镰刀菌分离株（包括引起葫芦维管枯萎病的五种特殊镰刀菌）的遗传相似性。用三种酶（PstI、HindIII 和 EcoRI）消化总 DNA，进行 Southern 印迹，并与 F.o. 杂交。 niveum mtDNA 多探针 (pFON2a-pFON8b)。通过使用平均值（UPGMA）和简约分析的未加权配对组方法检查mtDNA片段的存在或不存在。总共检测到14个mtDNA RFLP组。每个特殊形式中都有独特的 RFLP；然而，每种特殊形式通常出现最频繁的一种模式。两个 RFLP 组（RFLPG-fspI 和 IX）分别出现在四种和两种特殊形式中。 RFLP 单倍型在 F. o. 中最常见。除 F. o. 外，niveum (RFLPG-fspI) 也出现在来自所有其他特殊福菌的一个或多个分离株中。丝瓜，存在于北美、欧洲和亚洲的分离株中。 UPGMA 生成的遗传距离表明 F. o. niveum 是最不多样化的特殊形式，而 F. o.黄瓜是最多样化的。然而，聚类分析和简约分析均表明，葫芦科植物中的所有尖孢镰刀菌都是密切相关的，并且在某些情况下，不同尖孢镰刀菌的分离株在遗传上比相同尖孢镰刀菌的分离株更为相似。
Thirty-nine isolates of Fusarium oxysporum encompassing five formae speciales causing vascular wilt in cucurbits were examined for genetic similarity by restriction fragment length polymorphisms (RFLP) analysis of mitochondrial DNA (mtDNA). Total DNA was digested with three enzymes (PstI, HindIII, and EcoRI), Southern blotted, and hybridized with a F. o. niveum mtDNA polyprobe (pFON2a-pFON8b). The presence or absence of mtDNA fragments was examined by the unweighted-paired-group method using averages (UPGMA) and parsimony analysis. A total of 14 mtDNA RFLP groups were detected. Within each forma specialis there were unique RFLPs; however, one pattern generally occurred most frequently for each forma specialis. Two RFLP groups (RFLPG-fspI and IX) occurred in four and two formae speciales, respectively. The RFLP haplotype most common in F. o. niveum (RFLPG-fspI) also occurred in one or more isolates from every other formae speciales except F. o. luffae and was present in isolates from North America, Europe, and Asia. Genetic distances generated by UPGMA suggest that F. o. niveum was the least diverse forma specialis, while F. o. cucumerinum was the most diverse. However, both cluster analysis and parsimony analysis indicated that all of F. oxysporum formae speciales in the cucurbits are closely related and, in some cases, isolates of different formae speciales were genetically more similar than isolates of the same forma specialis.