Chemical and biological characteristics of hydrolysate of crucian carp swim bladder: Focus on preventing ulcerative colitis
Chemical and biological characteristics of hydrolysate of crucian carp swim bladder: Focus on preventing ulcerative colitis
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DOI：
10.1016/j.jff.2020.104256
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发表时间：
2020-12-01
影响因子：
5.6
通讯作者：
Lu, Ya-Ping
中科院分区：
文献类型：
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作者：
Dai, Chen;Dai, Lin;Lu, Ya-Ping
溃疡性结肠炎（UC）的发病率在世界范围内呈上升趋势。目前，尚无有效的治疗方法。找到一种以食物为基础的预防UC的方法是很重要的。本研究采用模拟胃肠消化法制备了鲫鱼鱼鳔水解物（HCSB）。采用纳米液相色谱-杂化线性离子阱轨道阱质谱联用技术对HCSB中的178个多肽进行了鉴定。在一个过氧化氢诱导的肠上皮细胞系（IEC-6）氧化应激模型中，HCSB通过增加抗氧化酶活性和降低细胞内ROS含量来保护IEC-6细胞。HCSB还能抑制脂多糖诱导的IEC-6细胞炎症。体内研究表明，通过降低髓过氧化物酶活性、促炎细胞因子的产生和抑制nf - κ B通路的激活，HCSB预防性给药可以减轻小鼠葡聚糖硫酸钠诱导UC的严重程度。这些结果表明HCSB具有预防溃疡性结肠炎的潜力。
The incidence of ulcerative colitis (UC) is increasing worldwide. Currently, no curative treatment is available. Finding a food-based method for prevention of UC would be significant. In this study, the hydrolysate of crucian carp swim bladder (HCSB) was prepared using a simulated gastrointestinal digestion. 178 peptides in HCSB were identified by nano liquid chromatography coupled with hybrid linear ion trap Orbitrap mass spectrometry. In a model of hydrogen peroxide-induced oxidative stress in an intestinal epithelial cell line (IEC-6), HCSB protects IEC-6 cells by increasing antioxidant enzyme activity and reducing the intracellular ROS content. HCSB also represses lipopolysaccharide-induced inflammation of IEC-6 cells. In vivo studies showed that prophylactic administration of HCSB reduces the severity of dextran sodium sulfate-induced UC in mice by reducing myeloperoxidase activity, production of proinflammatory cytokines, and by inhibiting activation of the NF-kappa B pathway. These results indicate that HCSB has the potential to prevent ulcerative colitis.