Nucleotide sequencing and transcriptional mapping of the Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus p10 gene.
Nucleotide sequencing and transcriptional mapping of the Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus p10 gene.
复制标题
Orgyia pseudotsugata 多衣壳核多角体病毒 p10 基因的核苷酸测序和转录图谱。
DOI：
10.1016/0042-6822(86)90019-x
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发表时间：
1986
期刊：
影响因子：
3.7
通讯作者：
Beaudreau,GS
中科院分区：
文献类型：
--
作者：
Leisy,DJ;Rohrmann,GF;Nesson,M;Beaudreau,GS
A32P-labeled cloned DNA fragment (AcMNPVHindIII-Q) containing one of the repeated sequences and a portion of the p10 gene fromAutographa californicamulticapsid nuclear polyhedrosis virus (AcMNPV) was used to probe Southern blots containing restriction endonuclease digests ofOrgyia pseudotsugatamuticapsid nuclear polyhedrosis virus (OpMNPV) DNA. A single 3.6-kb fragment, OpMNPVHindIII-Q, was hybridized. The OpMNPVHindIII-Q fragment was cloned into pUC-18, mapped with restriction endonucleases, and reprobed with the AcMNPVHindIII-Q fragment. A small region of ca. 700 bp, near the left end of the cloned fragment, was cross-hybridized. DNA sequencing in this region revealed an open reading frame of 279 by which shares detectable homology with the p10 gene of AcMNPV. The sequences downstream from the p10 gene in both viruses also contain long open reading frames which share homology. Northern blot analysis of RNA from OpMNPV infectedO. leucostigmacells was used to define the temporal and spatial organization of transcripts from this region. S1 analysis of both termini of the major p10 mRNA indicates nontranslated regions of 52–53 bases at the 5′ end and 175 bases at the 3′ end. The 5′-mRNA start site was located within a 12-nucleotide sequence which is conserved in all late hyperexpressed baculovirus genes.
