ALTERATIONS IN CYTOTOXIC AND PHENOTYPIC SUBSETS OF NATURAL-KILLER-CELLS IN ACQUIRED-IMMUNE-DEFICIENCY-SYNDROME (AIDS)
ALTERATIONS IN CYTOTOXIC AND PHENOTYPIC SUBSETS OF NATURAL-KILLER-CELLS IN ACQUIRED-IMMUNE-DEFICIENCY-SYNDROME (AIDS)
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DOI：
10.1007/bf00915420
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发表时间：
1987-01-01
影响因子：
9.1
通讯作者：
BEALL, G
中科院分区：
文献类型：
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作者：
PLAEGERMARSHALL, S;SPINA, CA;BEALL, G
使用一组自然杀伤（NK）敏感靶细胞（包括独特的单纯疱疹病毒感染的Raji细胞靶细胞）进行细胞毒性功能检测，并结合双色流式细胞术进行表型细胞分析，以表征NK系统。研究中纳入的受试者有感染获得性免疫缺陷综合征（AIDS）、人类免疫缺陷病毒（HIV）的风险。NK细胞功能的普遍缺陷与疾病表现在时间上相关，如艾滋病和艾滋病相关综合征（ARC）患者的NK细胞溶解功能缺陷所证明。健康的高危受试者，包括HIV血清阳性受试者，表现出强大的NK细胞功能。表型分析表明，正常比例的NK相关的CD 16+（Leu 11）Leu 7-和CD 16+（Leu 11）Leu 7+淋巴细胞亚群维持整个HIV感染的临床进展。然而，在AIDS、ARC和健康高危受试者（包括HIV血清阴性受试者）中，CD 8+（Leu 2）Leu 7+亚群细胞的比例和数量增加。这些结果是一致的，在艾滋病NK系统的质量缺陷，可能继发于CD 4-细胞耗竭和随之而来的缺乏必要的辅助因子。CD 8+（Leu 2）/Leu 7+细胞的升高不仅仅是HIV感染的结果，可能是对病毒和/或其他抗原刺激的一般反应。
Testing of cytotoxic function using a panel of natural killer (NK)-sensitive target cells, including a unique herpes simplex virus-infected Raji-cell target, was performed in conjunction with phenotypic cell analysis by dual-color flow cytometry to characterize the NK system. Subjects included in the study were at risk for or infected with the etiologic agent of the acquired immune deficiency syndrome (AIDS), human immunodeficiency virus (HIV). A generalized defect in NK function was temporally correlated with disease manifestations, as evidenced by deficient NK lytic function in patients with AIDS and AIDS related complex (ARC). Healthy at-risk subjects, including those seropositive for HIV, exhibited robust NK-cell function. Phenotypic analysis revealed that normal proportions of the NK-associated CD16+ (Leu11) Leu7- and CD16+(Leu11)Leu7+ lymphocyte subsets were maintained throughout the clinical progression of HIV infection. However, the proportion and numbers of cells of the CD8+(Leu2)Leu7+ subset were increased in AIDS, ARC, and healthy at-risk subjects, including those seronegative for HIV. These results are consistent with a qualitative defect in the NK system in AIDS, perhaps secondary to CD4-cell depletion and a concomitant lack of essential accessory factors. The elevation in CD8+(Leu2)/Leu7+ cells is not solely the result of HIV infection and may be a general response to viruses and/or other antigenic stimulation.