CHROMOSOMAL ANALYSIS OF SPERM FROM MEN WITH IDIOPATHIC INFERTILITY USING SPERM KARYOTYPING AND FLUORESCENCE IN-SITU HYBRIDIZATION
CHROMOSOMAL ANALYSIS OF SPERM FROM MEN WITH IDIOPATHIC INFERTILITY USING SPERM KARYOTYPING AND FLUORESCENCE IN-SITU HYBRIDIZATION
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DOI：
10.1016/s0015-0282(16)57859-5
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发表时间：
1995-10-01
影响因子：
6.7
通讯作者：
MARTIN, RH
中科院分区：
文献类型：
--
作者：
MOOSANI, N;PATTINSON, HA;MARTIN, RH
目的：确定患有少精子症、弱精子症或畸形症的特发性不育男性是否会增加将染色体异常传递给后代的风险。设计：采用人类精子-金黄地鼠卵母细胞融合系统检测5例躯体正常不育男性的精子染色体补体，并检测染色体1、12、地点：不育男性和正常捐献者为学术研究环境中的志愿者。患者：在关注其不育症的患者中招募5名精液特征合适的男性。干预：将精子与金黄地鼠卵母细胞融合，形成精子染色体或精子核，进行荧光原位杂交。主要观察指标：精子核型评价结构和数量异常，荧光原位杂交分析二体频率。结果：不育男性精子核型分析显示精子数目异常和总异常的发生率增加。对精子核进行荧光原位杂交分析，发现染色体I和XY二倍体频率显著增加。结论：不育男性精子中可能存在染色体异常。
Objective: To determine if idiopathic infertile men having oligozoospermia, asthenozoospermia, or teratozoospermia have an elevated risk of transmitting chromosomal abnormalities to their offspring.Design: Sperm chromosomal complements from five somatically normal infertile men were assayed using the human sperm-hamster oocyte fusion system and the disomy frequencies for chromosomes 1, 12, and the sex chromosomes were determined using fluorescence in situ hybridization.Setting: Infertile men and normal donors were volunteers in an academic research environment.Patients: Five men with the appropriate semen characteristics were recruited among patients concerned about their infertility.Interventions: Sperm fused with hamster oocytes resulting in sperm chromosomes or the sperm nuclei were prepared for fluorescence in situ hybridization.Main Outcome Measure: Structural and numerical abnormalities assessed by sperm karyotypes and the disomy frequency determined by fluorescence in situ hybridization analysis.Results: The infertile men showed increased frequencies of numerical abnormalities and total abnormalities as determined by sperm karyotyping. Analysis of sperm nuclei by fluorescence in situ hybridization indicated a significant increase in the frequency of disomy for chromosome I and XY disomy.Conclusions: Sperm from infertile men may contain an increased frequency of chromosomal abnormalities.