Mussel Coating Protein-Derived Complex Coacervates Mitigate Frictional Surface Damage.
Mussel Coating Protein-Derived Complex Coacervates Mitigate Frictional Surface Damage.
复制标题
贻贝涂料蛋白质衍生的复合物凝聚会减轻摩擦表面损伤。
DOI：
10.1021/acsbiomaterials.5b00252
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发表时间：
2015-11-09
影响因子：
5.8
通讯作者：
Waite JH
中科院分区：
文献类型：
--
作者：
Miller DR;Das S;Huang KY;Han S;Israelachvili JN;Waite JH
摩擦在生物材料界面功能性能中的作用被广泛认为是关键和复杂的，但人们对此知之甚少。为了更好地了解摩擦力，我们调查了生活在高能冲浪栖息地的贻贝的Holdfast或byssus的自然适应情况。角质层作为角质骨最外层的覆盖物，因其对剪切摩擦磨损的适应性而特别值得关注。在这项研究中，我们用透明质酸(HA)凝聚了角质层关键成分贻贝足部蛋白1、MFP-1[(1)加州贻贝mcfp-1、(2)rmfp-1和(3)rmfp-1-多巴]的三种变体之一，并研究了这些凝聚物在剪切过程中对表面(云母)的磨损保护能力。天然MCFP-1/HA胶凝剂具有中等的摩擦系数(μ∼0.3)，但对云母具有良好的耐磨性，且不会受到外加载荷的破坏，F⊥高达300mN(压力，P>；2 Mpa)。重组RMFP-1/HA凝聚体的摩擦系数与天然蛋白凝聚体相当(μ∼为0.3)，但耐磨性明显低于天然蛋白凝聚体(F⊥>；60mN时的损伤)。加入表面粘结剂3，4-二羟基苯丙氨酸(DOPA)后，RMFP-1/HA胶粘剂的耐磨性提高了5倍。我们提出了一种依赖于DOPA的磨损保护机制来解释不同凝聚体之间的磨损保护差异。我们的结果显示，凝聚剂在需要粘合、润滑和磨损保护的应用中具有巨大的未开发潜力。这些应用包括人工关节、隐形眼镜、牙科密封剂以及头发和皮肤护发素。
The role of friction in the functional performance of biomaterial interfaces is widely reckoned to be critical and complicated but poorly understood. To better understand friction forces, we investigated the natural adaptation of the holdfast or byssus of mussels that live in high-energy surf habitats. As the outermost covering of the byssus, the cuticle deserves particular attention for its adaptations to frictional wear under shear. In this study, we coacervated one of three variants of a key cuticular component, mussel foot protein 1, mfp-1 [(1) Mytilus californianus mcfp-1, (2) rmfp-1, and (3) rmfp-1-Dopa], with hyaluronic acid (HA) and investigated the wear protection capabilities of these coacervates to surfaces (mica) during shear. Native mcfp-1/HA coacervates had an intermediate coefficient of friction (μ ∼0.3) but conferred excellent wear protection to mica with no damage from applied loads, F⊥, as high as 300 mN (pressure, P, > 2 MPa). Recombinant rmfp-1/HA coacervates exhibited a comparable coefficient of friction (μ ∼0.3); however, wear protection was significantly inferior (damage at F⊥ > 60 mN) compared with that of native protein coacervates. Wear protection of rmfp-1/HA coacervates increased 5-fold upon addition of the surface adhesive group 3,4-dihydroxyphenylalanine, (Dopa). We propose a Dopa-dependent wear protection mechanism to explain the differences in wear protection between coacervates. Our results reveal a significant untapped potential for coacervates in applications that require adhesion, lubrication, and wear protection. These applications include artificial joints, contact lenses, dental sealants, and hair and skin conditioners.