Nucleotide sequence of reovirus genome segment S3, encoding non-structural protein sigma NS.
Nucleotide sequence of reovirus genome segment S3, encoding non-structural protein sigma NS.
复制标题
呼肠孤病毒基因组片段 S3 的核苷酸序列，编码非结构蛋白 sigma NS。
DOI：
10.1093/nar/11.18.6399
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发表时间：
1983
影响因子：
14.9
通讯作者：
Y. Furuichi
中科院分区：
文献类型：
--
作者：
M. Richardson;Y. Furuichi
该报告描述了人类呼肠孤病毒（Dearing 株）基因组片段 S3 的完整核苷酸序列。先前的研究表明，该 RNA 编码主要的非结构病毒多肽 sigma NS，这是一种结合 ssRNA 的蛋白质 (Huisman & Joklik, Virology 70, 411-424, 1976)，并具有聚 (C) 依赖性聚 (G) 聚合酶活性 (Gomatos 等人, J. Virol. 39, 115-124, 1981)。该基因组片段由 1,198 个核苷酸组成，并拥有一个开放阅读框，从第一个 AUG 三联体（残基 28-30）延伸出 366 个密码子。之前确定的基因组片段S3的正链和基因组片段S2的正链之间不存在显着的序列同源性(Cashdollar等人，PNAS 79, 7644-7648, 1982)。然而，S3 RNA 具有显着的二元对称性和可能与 S2 RNA 杂交（delta G = -26 KCal/摩尔）的区域。根据预测的氨基酸序列，确定了 Sigma NS 蛋白可能的二级结构。讨论了呼肠孤病毒 RNA 和 sigma NS 的结构特征及其在病毒基因组组装中的作用。
This report describes the complete nucleotide sequence of human reovirus (Dearing strain) genome segment S3. Previous studies indicated that this RNA encodes the major non-structural viral polypeptide sigma NS, a protein that binds ssRNAs (Huisman & Joklik, Virology 70, 411-424, 1976) and has a poly(C)-dependent poly(G) polymerase activity (Gomatos et al., J. Virol. 39, 115-124, 1981). The genome segment consists of 1,198 nucleotides and possesses an open reading frame that extends 366 codons from the first AUG triplet (residues 28-30). There is no significant sequence homology between the plus strand of genome segment S3 and that of genome segment S2 determined previously (Cashdollar et al., PNAS 79, 7644-7648, 1982). However, S3 RNA has significant dyad symmetry and regions that can potentially hybridize (delta G = -26 KCal/mole) with S2 RNA. From the predicted amino acid sequence a possible secondary structure for sigma NS protein was determined. Structural features of reovirus RNA and sigma NS are discussed in relation to their role(s) in viral genome assembly.