OLIGOCLONAL EXPANSION AND CD1 RECOGNITION BY HUMAN INTESTINAL INTRAEPITHELIAL LYMPHOCYTES
OLIGOCLONAL EXPANSION AND CD1 RECOGNITION BY HUMAN INTESTINAL INTRAEPITHELIAL LYMPHOCYTES
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DOI：
10.1126/science.1716785
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发表时间：
1991-09-20
期刊：
影响因子：
56.9
通讯作者：
BLUMBERG, RS
中科院分区：
文献类型：
--
作者：
BALK, SP;EBERT, EC;BLUMBERG, RS
从空肠建立人肠上皮内淋巴细胞（IEL）T细胞系，以表征该群体表达的α-β T细胞抗原受体（TCR）的结构和功能。单侧聚合酶链反应（PCR）扩增克隆和定量PCR扩增的TCR链从细胞系和新鲜的IEL证明，IEL寡克隆。IEL T细胞系表现出CD 1特异性细胞毒性，并且优势IEL T细胞克隆是CD 1c特异性的。因此，人空肠上皮内淋巴细胞是寡克隆的，并识别CD 1基因家族的成员。
A human intestinal intraepithelial lymphocyte (IEL) T cell line was established from jejunum to characterize the structure and function of the alpha-beta T cell antigen receptors (TCRs) expressed by this population. Single-sided polymerase chain reaction (PCR) amplification cloning and quantitative PCR amplification of the TCR chains from the cell line and from fresh IELs demonstrated that IELs were oligoclonal. The IEL T cell line exhibited CD1-specific cytotoxicity and a dominant IEL T cell clone was CD1c-specific. Thus, human jejunal intraepithelial lymphocytes are oligoclonal and recognize members of the CD1 gene family.