beta-Carotene-mediated inhibition of a DNA adduct induced by 7,12-dimethylbenz(a)anthracene and 7-hydroxymethyl-12-methylbenz(a)anthracene in mouse mammary gland in vitro.
beta-Carotene-mediated inhibition of a DNA adduct induced by 7,12-dimethylbenz(a)anthracene and 7-hydroxymethyl-12-methylbenz(a)anthracene in mouse mammary gland in vitro.
复制标题
β-胡萝卜素介导的对小鼠乳腺中 7,12-二甲基苯并（a）蒽和 7-羟甲基-12-甲基苯（a）蒽诱导的 DNA 加合物的抑制作用。
DOI：
10.1016/0959-8049(92)90470-m
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Banerjee,MR
中科院分区：
文献类型：
--
作者：
Das,SK;Jia,TZ;Bandyopadhyay,AM;Banerjee,MR
The influence of β-carotene on the formation of DNA-adducts induced by 7,12-dimethylbenz(a)anthracene (DMBA) and 7-hydroxymethyl-12-methylbenz(a)anthracene (7-OHM-12-MBA) during transformation of mouse mammary cells in organ culture was analysed. Treatment with β-carotene (10−8–10−5mol/l) caused inhibition (48.8–94.4%) of an adduct (VI), which was detectable in DNA samples from DMBA-treated mammary glands. Out of six adducts, derived from further analysis of DNA samples from 7-OHM-12-MBA-treated glands, adduct f eluted in the same fraction as adduct (VI), indicating these adducts were analogous. Likewise, adduct f was also inhibited by β-carotene. Boronate chromatographic analysis revealed this particular adduct was a syn-dihydrodiol epoxide product. Adduct inhibition was detectable both at the start and after DMBA treatment. α-Tocopherol and canthaxanthin were ineffective in inhibiting adducts. It is reasonable to conclude that β-carotene-mediated modification of adducts is associated with the inhibition of a syn-adduct, which is derived from further metabolism of a 7-OHM-12-MBA intermediate.
