Immunoglobulin G enzyme-linked immunosorbent assay using truncated nucleoproteins of Reston Ebola virus
Immunoglobulin G enzyme-linked immunosorbent assay using truncated nucleoproteins of Reston Ebola virus
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DOI：
10.1017/s0950268803008264
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发表时间：
2003-06-01
影响因子：
4.2
通讯作者：
Morikawa, S
中科院分区：
文献类型：
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作者：
Ikegami, T;Saijo, M;Morikawa, S
我们用莱斯顿埃博拉病毒(EBO-R)和扎伊尔埃博拉病毒(EBO-Z)的部分重组核蛋白(RNP)建立了免疫球蛋白G(IgG)酶联免疫吸附试验(EL ISA)。在免疫球蛋白酶联免疫吸附试验中，我们检测了10份自然感染EBO-R的食蟹猴血清对部分RNP的反应。所有血清均与EBO-R和EBO-Z的RNP的C端半部分发生反应。大多数血清与RAC(氨基酸360-739)、RDelta6(aA451-551)和/或RDelta8(aA631-739)的稀释度高于对EBO-Z截短RNPs的反应。结果表明，该方法可用于EBO-R特异性抗体的检测，并有可能用于EBO-R感染与其他亚型的鉴别。
We developed an immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA), using partial recombinant nucleoproteins (rNP) of Reston Ebola virus (EBO-R) and Zaire Ebola virus (EBO-Z). We examined the reaction of 10 sera from cynomolgus macaques naturally infected with EBO-R to each of the partial rNP in the IgG ELISA. All the sera reacted to the C-terminal halves of the rNP of both EBO-R and EBO-Z. Most of the sera reacted to the RAC (amino acid (aa) 360-739), and RDelta6 (aa 451-551) and/or RDelta8 (aa 631-739) at a higher dilution than to the corresponding truncated rNPs of EBO-Z. The results indicate that this IgG ELISA is useful for detecting EBO-R specific antibody, and may have a potential to discriminate EBO-R infection from other subtypes.