Genetic control of the immune response to staphylococcal nuclease. VII. Role of non-H2-linked genes in the control of the anti-nuclease antibody response.
Genetic control of the immune response to staphylococcal nuclease. VII. Role of non-H2-linked genes in the control of the anti-nuclease antibody response.
复制标题
对葡萄球菌核酸酶免疫反应的遗传控制。 vii。非H2连锁基因在控制抗核抗体反应中的作用。
DOI：
10.1084/jem.147.2.396
复制
发表时间：
1978-02-01
影响因子：
15.3
通讯作者：
Sachs, D H
中科院分区：
文献类型：
--
作者：
Pisetsky, D S;Berzofsky, J A;Sachs, D H
研究了非H-2连锁基因在控制葡萄球菌核酸酶抗体反应中的作用。用完全弗氏佐剂核酸酶免疫3wk后，A/J(H-2a)品系小鼠产生的抗体滴度显著高于B10.A(H-2(A))品系小鼠，而A.BY(H-2(B))和B10(H-2(B))品系小鼠产生的抗体效价几乎检测不到。在超免疫条件下，A/J和A/BY小鼠的抗体滴度达到了相同的峰值水平，均比B10.A和B10小鼠高出数倍。通过评估与两个核酸酶片段的结合来分析抗体的特异性，表明相同H-2单倍型的菌株之间存在相似之处。这些结果表明，尽管H-2-1连锁基因决定了3wk时的初始反应性和针对核酸酶分子上不同抗原决定因素的抗体的相对比例，但非H-2连锁基因决定了高免疫反应的总体程度。对核酸酶片段(1-126)的亲和力测定表明，菌株B10和B10产生的抗体亲和力是菌株A和A/J的7-10倍。(B10.A×A/J)×B10.A回交，抗体水平独立于Ig-1(E)C(H)异型和A/J抗核酸酶独特型。因此，一个既不与H-2也不与重链结构基因连锁的基因(S)似乎控制着对核酸酶分子上抗原决定簇的聚合反应，而与这些抗体的亚特异性或它们的独特型无关。
The role of non-H-2-linked genes in the control of the antibody response to staphylococcal nuclease has been investigated. 3 wk after immunization with nuclease in complete Freund's adjuvant, strain A/J (H-2 a) mice produced significantly higher titers of antibody than strain B10.A (H-2(a)) mice, whereas mice of strains A.BY (H-2(b)) and B10 (H-2(b)) produced barely detectable titers. With hyperimmunization, A/J and A.BY mice reached the same peak levels for antibody titers, both severalfold higher than those reached by B10.A and B10 mice. Analysis of the specificity of antibodies by assessment of binding to two fragments of nuclease showed similarities between strains of the same H-2 haplotype. These results suggest that although H-2-1inked genes determined initial responsiveness at 3 wk and the relative proportions of antibodies directed toward different antigenic determinants on the nuclease molecule, non-H-2-linked genes determined the overall magnitude of the hyperimmuneresponse. Measurement of the affinity of the antibodies to the nuclease fragment (1-126) showed that strains B10 and B10.A produced antibodies with 7- to 10-fold higher affinity than comparable antibodies from strains A.BY and A/J. In a backcross of (B10.A × A/J) × B10.A, the level of antibody segregated independently of the Ig-1(e) C(H) allotype and the A/J anti-nuclease idiotypes. Thus, a gene(s) linked to neither H-2 nor heavy chain structural genes appears to control the aggregate response to antigenic determinants on the nuclease molecule independent of subspecificities of these antibodies or their idiotype.