EARLY B-CELL PRECURSORS IN SCID MICE - NORMAL NUMBERS OF CELLS TRANSFORMABLE WITH ABELSON MURINE LEUKEMIA-VIRUS (A-MULV)
EARLY B-CELL PRECURSORS IN SCID MICE - NORMAL NUMBERS OF CELLS TRANSFORMABLE WITH ABELSON MURINE LEUKEMIA-VIRUS (A-MULV)
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DOI：
10.1016/0008-8749(88)90017-2
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发表时间：
1988-04-15
影响因子：
4.3
通讯作者：
PHILLIPS, RA
中科院分区：
文献类型：
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作者：
FULOP, GM;BOSMA, GC;PHILLIPS, RA
scid mice lack detectable B and T lymphocytes; there are no typical pre-B cells as defined by c.mu. and surface markers in their bone marrow and their thymus contains only 1% of the normal number of cells. In these characters scid mice seem to lack lymphoid stem cells. However, some mice have detectable serum immunoglobulin and others develop thymomas; both observations indicate that the block in lymphoid development is not absolute. To determine whether scid mice have any B-cell precursors, we looked for pre-B cell by their ability to be transformed by Abelson murine leukemia virus (A-MuLV). Surprisingly, scid mice contain as many B-cell precursors transformable with A-MuLV as normal control mice. Cell-surface markers specific for pre-B and B cells were detected on the A-MuLV-transformed bone marrow cells of both scid and normal mice, indicating that the A-MuLV-transformed cells belong to the B lineage. Interestingly, the same surface markers were undetectable on nontransformed scid bone marrow cells. We conclude from these results that scid mice have normal numbers of early B-cell precursors but that their differentiation into functional B cells is severely impaired.
