Functional analysis of the cytochrome P450 monooxygenase gene bcbot1 of Botrytis cinerea indicates that botrydial is a strain-specific virulence factor
Functional analysis of the cytochrome P450 monooxygenase gene bcbot1 of Botrytis cinerea indicates that botrydial is a strain-specific virulence factor
复制标题
DOI：
10.1094/mpmi-18-0602
复制
发表时间：
2005-06-01
影响因子：
3.5
通讯作者：
Tudzynski, P
中科院分区：
文献类型：
--
作者：
Siewers, V;Viaud, M;Tudzynski, P
番茄灰霉病菌(Botrytis Cinerea)在大量双子叶植物和观赏植物上引起灰霉病。寄主组织的定植伴随着植物细胞在菌丝生长之前的迅速死亡，这可能是由于分泌非特异性的植物毒素，如倍半萜葡萄糖醇。虽然到目前为止测试的所有致病菌株都被证明分泌葡萄球菌，尽管这种毒素引起的坏死性病变与真菌感染类似，但葡萄孢子菌在感染过程中的作用迄今尚未阐明。在这里，我们描述了编码P450单加氧酶的bebot1的功能特征，并提供了它参与盆腔途径的证据，即它是第一个被发现的盆腔生物合成基因。我们发现bcbot1在PLANTA中表达，并且在体外和在PLANTA中的表达受异源三聚体GTP结合蛋白BCG1的阿尔法亚单位控制。在三个标准菌株中bcbot1的缺失表明，对毒力的影响(在几种寄主植物上)是依赖于菌株的；只有其中一个菌株(T4)的缺失会导致毒力降低。
The micrographic phytopathogen Botrytis cinerea causes gray mold diseases in a large number of dicotyledonous crop plants and ornamentals. Colonization of host tissue is accompanied by rapid killing of plant cells ahead of the growing hyphen, probably caused by secretion of nonspecific phytotoxins, e.g., the sesquiterpene botrydial. Although all pathogenic strains tested so far had been shown to secrete botrydial and although the toxin causes comparable necrotic lesions as infection by the fungus, the role of botrydial in the infection process has not been elucidated so far. Here, we describe the functional characterization of bebot1, encoding a P450 monooxygenase and provide evidence that it is involved in the botrydial pathway, i.e., it represents the first botrydial biosynthetic gene identified. We show that bcbot1 is expressed in planta and that expression in vitro and in planta is controlled by an alpha-subunit of a heterotrimeric GTP-binding protein, BCG1. Deletion of bcbot1 in three standard strains of B. cinerea shows that the effect on virulence (on several host plants) is strain-dependent; only deletion in one of the strains (T4) led to reduced virulence.