TOTAL NUMBERS OF VARIOUS CELL-TYPES IN RAT CEREBELLAR CORTEX ESTIMATED USING AN UNBIASED STEREOLOGICAL METHOD
TOTAL NUMBERS OF VARIOUS CELL-TYPES IN RAT CEREBELLAR CORTEX ESTIMATED USING AN UNBIASED STEREOLOGICAL METHOD
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DOI：
10.1016/0006-8993(93)90881-m
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发表时间：
1993-04-23
期刊：
影响因子：
2.9
通讯作者：
MOLLER, A
中科院分区：
文献类型：
--
作者：
KORBO, L;ANDERSEN, BB;MOLLER, A
本文提出了一种新的有效的体视学方法来估计大鼠小脑皮质不同类型细胞的总数。这些细胞被细分为以下类别：浦肯野细胞、颗粒细胞、高尔基细胞、颗粒层中的胶质细胞、Bergmann胶质细胞以及分子层中的神经元和胶质细胞。该方法已被用于估计与对照组相比，暴露于200、400和800 mg/kg/天剂量甲苯经口给药12周的大鼠中某些细胞类型的总数。在接触和未接触动物之间未发现统计学显著差异。所描述的方法可用于许多生物学和实验研究。随着新的体视学方法的使用，有可能在比以前所需的短得多的时间内获得总细胞数的精确估计。这使得有可能通过增加处理的案件数量来提高最终结果的可靠性。
A new and efficient stereological method for estimating the total number of the different cell types in rat cerebellar cortex is presented. The cells have been subdivided into the following categories: Purkinje cells, granule cells, Golgi cells, glial cells in the granular layer, Bergmann glial cells and neurons and glial cells in the molecular layer. The method has been used to estimate the total number of some of the cell types in rats exposed to orally administered toluene at doses of 200, 400 and 800 mg/kg/day for 12 weeks compared with a control group. No statistically significant differences were found between the exposed and non-exposed animals. The described method can be used in a number of both biological and experimental studies. With the use of new stereological methods it is possible to get precise estimates of total cell numbers in a much shorter time than earlier required. This makes it possible to improve the reliability of the final result by increasing the number of cases processed.